Literature DB >> 28301252

Identification of Optimal Reference Genes for Normalization of RT-qPCR Data in Cancerous and Non-Cancerous Tissues of Human Uterine Cervix.

Shing Cheng Tan1, Mohd Pazudin Ismail2, Daniel Roza Duski3, Nor Hayati Othman4, Venkata Murali Krishna Bhavaraju5, Ravindran Ankathil1.   

Abstract

This study aimed to identify the most stably expressed reference genes from a panel of 32 candidate genes for normalization of reverse transcription-quantitative real-time polymerase chain reaction data in cancerous and non-cancerous tissues of human uterine cervix. Overall, PUM1, YWHAZ, and RPLP0 were identified as the most stably expressed genes in paired cancerous and non-cancerous tissues. The results were further stratified by the state of malignancy of the tissues, histopathological type of the cancer, and the human papillomavirus-type.

Entities:  

Keywords:  Endogenous control genes; gene expression; normalization; quantitative real-time PCR; reference genes; uterine cervical neoplasms

Mesh：

Year:  2017        PMID： 28301252     DOI： 10.1080/07357907.2017.1278767

Source DB:  PubMed          Journal:  Cancer Invest        ISSN： 0735-7907            Impact factor:   2.176


  6 in total

1.  Transcriptome Analysis of Gene Expression Provides New Insights into the Effect of Mild Therapeutic Hypothermia on Primary Human Cortical Astrocytes Cultured under Hypoxia.

Authors:  Mootaz M Salman; Philip Kitchen; M Nicola Woodroofe; Roslyn M Bill; Alex C Conner; Paul R Heath; Matthew T Conner
Journal:  Front Cell Neurosci       Date:  2017-12-14       Impact factor: 5.505

2.  Specific and reliable detection of Myosin 1C isoform A by RTqPCR in prostate cancer cells.

Authors:  Aleena A Saidova; Daria M Potashnikova; Anna V Tvorogova; Ivan V Maly; Wilma A Hofmann; Ivan A Vorobjev
Journal:  PeerJ       Date:  2018-11-20       Impact factor: 2.984

3.  Markers of Angiogenesis, Lymphangiogenesis, and Epithelial-Mesenchymal Transition (Plasticity) in CIN and Early Invasive Carcinoma of the Cervix: Exploring Putative Molecular Mechanisms Involved in Early Tumor Invasion.

Authors:  Olga Kurmyshkina; Pavel Kovchur; Ludmila Schegoleva; Tatyana Volkova
Journal:  Int J Mol Sci       Date:  2020-09-06       Impact factor: 5.923

4.  Reference gene selection for qRT-PCR analyses of luffa (Luffa cylindrica) plants under abiotic stress conditions.

Authors:  Min-Dong Chen; Bin Wang; Yong-Ping Li; Mei-Juan Zeng; Jian-Ting Liu; Xin-Ru Ye; Hai-Sheng Zhu; Qing-Fang Wen
Journal:  Sci Rep       Date:  2021-02-04       Impact factor: 4.379

5.  Bioinformatics Analysis of Genes and Pathways of CD11b+/Ly6Cintermediate Macrophages after Renal Ischemia-Reperfusion Injury.

Authors:  Dong Sun; Xin Wan; Bin-Bin Pan; Qing Sun; Xiao-Bing Ji; Feng Zhang; Hao Zhang; Chang-Chun Cao
Journal:  Curr Med Sci       Date:  2018-03-15

6.  Selection of endogenous control genes for normalising gene expression data derived from formalin-fixed paraffin-embedded tumour tissue.

Authors:  Tim A D Smith; Omneya A AbdelKarem; Joely J Irlam-Jones; Brian Lane; Helen Valentine; Becky A S Bibby; Helen Denley; Ananya Choudhury; Catharine M L West
Journal:  Sci Rep       Date:  2020-10-14       Impact factor: 4.379
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.