Literature DB >> 2830034

Improved mammalian vectors for high expression of G418 resistance.

K Katoh1, Y Takahashi, S Hayashi, H Kondoh.   

Abstract

Cell lines, such as those of teratocarcinoma and embryonic stem cells, fail to support high G418 resistance after transfection of neo vectors. To alleviate this, we modified pSV2-neo in two steps, first with tandem promoters of SV40 early genes and HSVtk, then by removing an improper met codon located immediately upstream of the authentic initiator codon in the mRNA sequence. In the final product, pSTneoB, the neo transcription unit has XhoI sites at both ends. pSTneoB yields G418-resistant transformants of teratocarcinoma cells at dramatically higher efficiencies than pSV2-neo. This vector extends the application of G418 selection in gene transfer to a wider range of cell types.

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Year:  1987        PMID： 2830034     DOI： 10.1247/csf.12.575

Source DB:  PubMed          Journal:  Cell Struct Funct        ISSN： 0386-7196            Impact factor:   2.212


  24 in total

1.  Cerebellar granule cell-specific and inducible expression of Cre recombinase in the mouse.

Authors:  M Tsujita; H Mori; M Watanabe; M Suzuki; J Miyazaki; M Mishina
Journal:  J Neurosci       Date:  1999-12-01       Impact factor: 6.167

2.  Homologous recombination at c-fyn locus of mouse embryonic stem cells with use of diphtheria toxin A-fragment gene in negative selection.

Authors:  T Yagi; Y Ikawa; K Yoshida; Y Shigetani; N Takeda; I Mabuchi; T Yamamoto; S Aizawa
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

3.  The PB1 subunit alone can catalyze cRNA synthesis, and the PA subunit in addition to the PB1 subunit is required for viral RNA synthesis in replication of the influenza virus genome.

Authors:  Y Nakagawa; K Oda; S Nakada
Journal:  J Virol       Date:  1996-09       Impact factor: 5.103

4.  Down-regulated expression of monocyte/macrophage major histocompatibility complex receptors in human and mouse monocytes by expression of their ligands.

Authors:  H Yamana; J Tashiro-Yamaji; M Hayashi; S Maeda; T Shimizu; N Tanigawa; K Uchiyama; T Kubota; R Yoshida
Journal:  Clin Exp Immunol       Date:  2014-10       Impact factor: 4.330

5.  Roles of semaphorin-6B and plexin-A2 in lamina-restricted projection of hippocampal mossy fibers.

Authors:  Hiroshi Tawarayama; Yutaka Yoshida; Fumikazu Suto; Kevin J Mitchell; Hajime Fujisawa
Journal:  J Neurosci       Date:  2010-05-19       Impact factor: 6.167

6.  Exploring new gene integration sites for gene knock-in by gene-trapping strategy.

Authors:  Isamu Nanchi; Yuki Yoshimura; Kazuomi Nakamura; Yusaku Masago; Tetsuya Ohbayashi; Tomohiko Okuda
Journal:  Transgenic Res       Date:  2015-03-31       Impact factor: 2.788

7.  Rapid and transient decrease of N-myc expression in retinoic acid-induced differentiation of OTF9 teratocarcinoma stem cells.

Authors:  K Kato; A Kanamori; H Kondoh
Journal:  Mol Cell Biol       Date:  1990-02       Impact factor: 4.272

8.  Interleukin 2 receptor beta chain expressed in an oligodendroglioma line binds interleukin 2 and delivers growth signal.

Authors:  Y Okamoto; S Minamoto; K Shimizu; H Mogami; T Taniguchi
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

9.  Disruption of epithelial cell-cell adhesion by exogenous expression of a mutated nonfunctional N-cadherin.

Authors:  T Fujimori; M Takeichi
Journal:  Mol Biol Cell       Date:  1993-01       Impact factor: 4.138

10.  Structure and regulation of the human interferon regulatory factor 1 (IRF-1) and IRF-2 genes: implications for a gene network in the interferon system.

Authors:  H Harada; E Takahashi; S Itoh; K Harada; T A Hori; T Taniguchi
Journal:  Mol Cell Biol       Date:  1994-02       Impact factor: 4.272
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