Literature DB >> 2829974

The mechanism of histone activation of the hepatic microsomal glucose-6-phosphatase system: a novel method to assay glucose-6-phosphatase activity.

J N Blair1, A Burchell.   

Abstract

The mechanism of activation of hepatic microsomal glucose-6-phosphatase (EC 3.1.3.9) by histone 2A has been investigated in both intact and disrupted microsomes. Histone 2A increased the Vmax and decreased the Km of glucose-6-phosphatase in intact microsomes but had no effect on glucose-6-phosphatase activity in disrupted microsomes. Histone 2A was shown to activate glucose-6-phosphatase in intact microsomes by disrupting the membrane vesicles and thereby allowing the direct measurement of the activity of the latent glucose-6-phosphatase enzyme. The study demonstrated that disrupting microsomes with histone 2A is an excellent method for directly assaying glucose-6-phosphatase activity as it poses none of the problems encountered with all of the previously used methods.

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Year:  1988        PMID： 2829974     DOI： 10.1016/0304-4165(88)90162-6

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  11 in total

1.  Analysis of human hepatic microsomal glucose-6-phosphatase in clinical conditions where the T2 pyrophosphate/phosphate transport protein is absent.

Authors:  R C Nordlie; H M Scott; I D Waddell; R Hume; A Burchell
Journal:  Biochem J       Date:  1992-02-01       Impact factor: 3.857

2.  Perinatal diagnosis of type 1c glycogen storage disease.

Authors:  A Burchell; I D Waddell; L Stewart; R Hume
Journal:  J Inherit Metab Dis       Date:  1989       Impact factor: 4.982

3.  The microsomal glucose-6-phosphatase enzyme of pancreatic islets.

Authors:  I D Waddell; A Burchell
Journal:  Biochem J       Date:  1988-10-15       Impact factor: 3.857

4.  Abnormal expression of glucose-6-phosphatase in preterm infants.

Authors:  R Hume; A Burchell
Journal:  Arch Dis Child       Date:  1993-02       Impact factor: 3.791

5.  11beta-hydroxysteroid dehydrogenase type 1 knockout mice show attenuated glucocorticoid-inducible responses and resist hyperglycemia on obesity or stress.

Authors:  Y Kotelevtsev; M C Holmes; A Burchell; P M Houston; D Schmoll; P Jamieson; R Best; R Brown; C R Edwards; J R Seckl; J J Mullins
Journal:  Proc Natl Acad Sci U S A       Date:  1997-12-23       Impact factor: 11.205

Review 6.  The glucose-6-phosphatase system.

Authors:  Emile van Schaftingen; Isabelle Gerin
Journal:  Biochem J       Date:  2002-03-15       Impact factor: 3.857

7.  Histone II-A stimulates glucose-6-phosphatase and reveals mannose-6-phosphatase activities without permeabilization of liver microsomes.

Authors:  J F St-Denis; B Annabi; H Khoury; G van de Werve
Journal:  Biochem J       Date:  1995-08-15       Impact factor: 3.857

8.  Changes in the glucose-6-phosphatase complex in hepatomas.

Authors:  Q Xiao; I Jaspers; E Matthew; M A Lea
Journal:  Mol Cell Biochem       Date:  1993-05-12       Impact factor: 3.396

9.  Astrocytic glucose-6-phosphatase and the permeability of brain microsomes to glucose 6-phosphate.

Authors:  R J Forsyth; K Bartlett; A Burchell; H M Scott; J A Eyre
Journal:  Biochem J       Date:  1993-08-15       Impact factor: 3.857

10.  Multiple transport protein defects in a patient with glycogen storage disease type 1: GSD 1b/1c beta.

Authors:  R A Hawkins; K R Kamath; H M Scott; A Burchell
Journal:  J Inherit Metab Dis       Date:  1995       Impact factor: 4.982
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