Amira M Gamal-Eldeen1, Dina Moustafa2, Sherien M El-Daly3, Mona A M Abo-Zeid4, Samira Saleh5, Menka Khoobchandani6, Kavita Katti6, Ravi Shukla7, Kattesh V Katti8. 1. Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt; Department of Biochemistry, National Research Centre, Cairo, Egypt. Electronic address: aeldeen7@yahoo.com. 2. Department of Pharmacology and Toxicology, Faculty of Pharmacy, October 6 University, 6 October City, Giza, Egypt. 3. Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt; Department of Medical Biochemistry, National Research Centre, Cairo, Egypt. 4. Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt; Genetics and Cytology Department, National Research Centre, Cairo, Egypt. 5. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, Egypt. 6. Department of Radiology, University of Missouri, Columbia, MO 65212, USA; Institute of Green Nanotechnology, University of Missouri, Columbia, MO 65212, USA. 7. Centre for Advanced Materials and Industrial Chemistry, School of Applied Sciences, Health Innovation Research Institute, RMIT University, Australia. 8. Department of Radiology, University of Missouri, Columbia, MO 65212, USA; Institute of Green Nanotechnology, University of Missouri, Columbia, MO 65212, USA; Research Reactor, University of Missouri, Columbia, MO 65212, USA; Department of Physics, University of Missouri, Columbia, MO 65212, USA; Department of Bioengineering, University of Missouri, Columbia, MO 65211, USA.
Abstract
BACKGROUND: In our previous work, we have extensively evaluated the physiochemical characteristics of Gum Arabic-encapsulated gold nanoparticles (GA-AuNPs; 15-18nm) and reported their effectiveness in stopping the tumor initiation via inhibiting the pre-neoplastic lesions in liver. OBJECTIVE: The rationale of this study is to detect the efficiency of using GA-AuNPs in photothermal application as a non-invasive technique against lung tumor. We investigated the cytotoxicity of GA-AuNPs on A549 cells, and then studied their apoptotic, anti-inflammatory, lipid peroxidation and anti-neovascular effect in in vivo model using a chemically-induced lung cancer in mice. The histopathological changes due to GA-AuNPs were investigated. RESULTS: In the presence of laser irradiation, GA-AuNPs had a considerable cytotoxicity against A549 cells. The treatment of lung tumor-bearing mice with GA-AuNPs followed by laser exposure enhanced the apoptotic pathway and this was obvious from the histopathological investigations and the elevations in cytochrome-c, death receptor 5 and the subsequent upregulation of caspase-3, we also reported a significant reduction in the levels of the inflammatory mediator TNF-α and the angiogenesis inducer VEGF. An induction of lipid peroxidation was also reported upon treatment with either GA or GA-AuNPs. CONCLUSION: GA-AuNPs showed no cytotoxicity in the absence of light, however the combination of GA-AuNPs with laser induced cell death in lung tumor tissues with a reduction in the inflammation and angiogenesis together with an elevation in lipid peroxidation, suggesting the potential use of these functionalized nanoparticles as a promising photothermal non-invasive treatment modality.
BACKGROUND: In our previous work, we have extensively evaluated the physiochemical characteristics of Gum Arabic-encapsulated gold nanoparticles (GA-AuNPs; 15-18nm) and reported their effectiveness in stopping the tumor initiation via inhibiting the pre-neoplastic lesions in liver. OBJECTIVE: The rationale of this study is to detect the efficiency of using GA-AuNPs in photothermal application as a non-invasive technique against lung tumor. We investigated the cytotoxicity of GA-AuNPs on A549 cells, and then studied their apoptotic, anti-inflammatory, lipid peroxidation and anti-neovascular effect in in vivo model using a chemically-induced lung cancer in mice. The histopathological changes due to GA-AuNPs were investigated. RESULTS: In the presence of laser irradiation, GA-AuNPs had a considerable cytotoxicity against A549 cells. The treatment of lung tumor-bearing mice with GA-AuNPs followed by laser exposure enhanced the apoptotic pathway and this was obvious from the histopathological investigations and the elevations in cytochrome-c, death receptor 5 and the subsequent upregulation of caspase-3, we also reported a significant reduction in the levels of the inflammatory mediator TNF-α and the angiogenesis inducer VEGF. An induction of lipid peroxidation was also reported upon treatment with either GA or GA-AuNPs. CONCLUSION:GA-AuNPs showed no cytotoxicity in the absence of light, however the combination of GA-AuNPs with laser induced cell death in lung tumor tissues with a reduction in the inflammation and angiogenesis together with an elevation in lipid peroxidation, suggesting the potential use of these functionalized nanoparticles as a promising photothermal non-invasive treatment modality.
Authors: Mohamed A Ashour; Waseem Fatima; Mohd Imran; Mohammed M Ghoneim; Sultan Alshehri; Faiyaz Shakeel Journal: Molecules Date: 2022-02-09 Impact factor: 4.411
Authors: Velaphi C Thipe; Kiandohkt Panjtan Amiri; Pierce Bloebaum; Alice Raphael Karikachery; Menka Khoobchandani; Kavita K Katti; Silvia S Jurisson; Kattesh V Katti Journal: Int J Nanomedicine Date: 2019-06-18