Literature DB >> 2829655

A preparative method for the enzymatic 5'-monophosphorylation of nucleosides.

D J Merkler1, V L Schramm.   

Abstract

A simple procedure to prepare a stable preparation of bovine liver adenosine kinase was developed. The adenosine kinase was used to phosphorylate a variety of purine nucleosides using ATP or [gamma-32P]ATP as the phosphoryl donor. A convenient scheme to purify the nucleotides was also developed.

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Year:  1987        PMID: 2829655     DOI: 10.1016/0003-2697(87)90145-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  1 in total

1.  A swift and facile radiosynthesis of [alpha-32P]-3'-azido-3'-deoxythymidine 5'-triphosphate (AZT triphosphate).

Authors:  P F Torrence; T Kovács
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

  1 in total

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