Nashwa Ismail Hashaad1, Rasha Mohamed Fawzy1, Abeer Ahmed Abo Elazem2, Mohamed Ibrahim Youssef3. 1. Department of Rheumatology, Rehabilitation and Physical Medicine, Benha University School of Medicine, Benha, Egypt. 2. Department of Microbiology and Immunology, Benha University School of Medicine, Benha, Egypt. 3. Deaprtment of Diagnostic Radiology, Benha University School of Medicine, Benha, 9Egypt.
Abstract
OBJECTIVE: This study aimed to investigate the relations between calreticulin (CRT) serum level and both disease activity and severity parameters in juvenile idiopathic arthritis (JIA). MATERIAL AND METHODS: In this study, 60 children with JIA and 50 age-and-sex-matched healthy subjects were enrolled. The assessment of the disease activity was done using juvenile arthritis disease activity score 27 (JADAS-27). The assessment of disease severity was done via gray-scale ultrasonography (US) and power Doppler US (PDUS). Enzyme-linked immunosorbent assay (ELISA) was used to assay the serum level of human CRT. RESULTS: The mean serum CRT levels in JIA patients was 8.6±1.2 ng/mL and showed a highly significant increase (p=0.001) as compared to the mean serum levels in the controls (5.02±0.77 ng/mL). There were statistically significant positive correlations between the serum CRT levels and disease duration, tender joint count, swollen joint count, visual analog scale, erythrocyte sedimentation rate, JADAS-27, C-reactive protein, rheumatoid factor titer, and ultrasonographic grading for synovitis and neovascularization. CONCLUSION: Elevated serum CRT levels in JIA patients and its correlations with JIA disease activity and severity parameters signified that CRT might be used as a novel biomarker for disease activity and severity in JIA.
OBJECTIVE: This study aimed to investigate the relations between calreticulin (CRT) serum level and both disease activity and severity parameters in juvenile idiopathic arthritis (JIA). MATERIAL AND METHODS: In this study, 60 children with JIA and 50 age-and-sex-matched healthy subjects were enrolled. The assessment of the disease activity was done using juvenile arthritis disease activity score 27 (JADAS-27). The assessment of disease severity was done via gray-scale ultrasonography (US) and power Doppler US (PDUS). Enzyme-linked immunosorbent assay (ELISA) was used to assay the serum level of humanCRT. RESULTS: The mean serum CRT levels in JIA patients was 8.6±1.2 ng/mL and showed a highly significant increase (p=0.001) as compared to the mean serum levels in the controls (5.02±0.77 ng/mL). There were statistically significant positive correlations between the serum CRT levels and disease duration, tender joint count, swollen joint count, visual analog scale, erythrocyte sedimentation rate, JADAS-27, C-reactive protein, rheumatoid factor titer, and ultrasonographic grading for synovitis and neovascularization. CONCLUSION: Elevated serum CRT levels in JIA patients and its correlations with JIA disease activity and severity parameters signified that CRT might be used as a novel biomarker for disease activity and severity in JIA.
Authors: Esperanza Naredo; Manuel Rodríguez; Cristina Campos; José Manuel Rodríguez-Heredia; Julio A Medina; Emilio Giner; Olga Martínez; Francisco Javier Toyos; Teresa Ruíz; Inmaculada Ros; Manuel Pujol; Xavier Miquel; Luz García; Juan José Aznar; Eugenio Chamizo; Manuel Páez; Pilar Morales; Amalia Rueda; Roser Tuneu; Hector Corominas; Juan José de Agustín; Carmen Moragues; Dolores Mínguez; Alfredo Willisch; Isabel González-Cruz; Angel Aragón; Gerardo Iglesias; Carlos Armas; Juan Pablo Valdazo; Carmen Vargas; Jaime Calvo-Alén; Antonio Juan-Mas; Georgina Salvador; Angels Puigdollers; Eva Galíndez; Noemi Garrido; José Salaberri; Enrique Raya; Meritxell Salles; César Díaz; José Luis Cuadra; Jesús Garrido Journal: Arthritis Rheum Date: 2008-04-15