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Literature DB >> 2829175

Tissue specificity, localization in brain, and cell-free translation of mRNA encoding the A3 isoform of Na+,K+-ATPase.

J W Schneider¹, R W Mercer, M Gilmore-Hebert, M F Utset, C Lai, A Greene, E J Benz.

Abstract

The isolation of multiple Na+,K+-ATPase cDNAs from rat brain has led to the discovery of a family of alpha-isoform genes. Using A1 (alpha), A2 (alpha+), and A3 (alpha III) Na+,K+-ATPase gene probes, we have analyzed the distribution of Na+,K+-ATPase mRNAs in adult and fetal rat tissues by RNA blot and hybridization histochemistry. A1 Na+,K+-ATPase mRNA was found ubiquitously among various tissues, with highest levels in transport epithelial and neural tissues. A2 mRNA was found in adult neural and muscle tissues, and A3 mRNA was found only in neural tissues and fetal heart muscle. Both A1 and A2 mRNAs were less abundant in fetal brain than in adult brain; in contrast, A3 mRNA was abundant at both stages. In situ mapping of brain areas that contain A3 mRNA suggests that this Na+,K+-ATPase isoenzyme is expressed predominantly by neural cells. Analysis of Na+,K+-ATPase proteins generated by cell-free translation of synthetic mRNAs suggests that the A3 protein has properties similar to A2 (alpha+).

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Year: 1988 PMID： 2829175 PMCID： PMC279529 DOI： 10.1073/pnas.85.1.284

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

32 in total

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Journal: Am J Physiol Date: 1985-05

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Journal: Am J Physiol Date: 1985-03

4. Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA.

Authors: J W Schneider; R W Mercer; M Caplan; J R Emanuel; K J Sweadner; E J Benz; R Levenson
Journal: Proc Natl Acad Sci U S A Date: 1985-09 Impact factor: 11.205

5. Insulin affects the sodium affinity of the rat adipocyte (Na+,K+)-ATPase.

Authors: J Lytton
Journal: J Biol Chem Date: 1985-08-25 Impact factor: 5.157

6. Enzymatic properties of separated isozymes of the Na,K-ATPase. Substrate affinities, kinetic cooperativity, and ion transport stoichiometry.

Authors: K J Sweadner
Journal: J Biol Chem Date: 1985-09-25 Impact factor: 5.157

7. Two different Na,K-ATPases in the optic nerve: cells of origin and axonal transport.

Authors: S C Specht; K J Sweadner
Journal: Proc Natl Acad Sci U S A Date: 1984-02 Impact factor: 11.205

8. Two classes of ouabain receptors in chick ventricular cardiac cells and their relation to (Na+,K+)-ATPase inhibition, intracellular Na+ accumulation, Ca2+ influx, and cardiotonic effect.

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9. Characterization of "high-affinity" [3H]ouabain binding in the rat central nervous system.

Authors: R Hauger; H M Luu; D K Meyer; F K Goodwin; S M Paul
Journal: J Neurochem Date: 1985-06 Impact factor: 5.372

10. Identification of two molecular forms of (Na+,K+)-ATPase in rat adipocytes. Relation to insulin stimulation of the enzyme.

Authors: J Lytton; J C Lin; G Guidotti
Journal: J Biol Chem Date: 1985-01-25 Impact factor: 5.157

23 in total

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Authors: J Melikian; F Ismail-Beigi
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2. Cell-specific expression of mRNAs encoding Na+,K(+)-ATPase alpha- and beta-subunit isoforms within the rat central nervous system.

Authors: A G Watts; G Sanchez-Watts; J R Emanuel; R Levenson
Journal: Proc Natl Acad Sci U S A Date: 1991-08-15 Impact factor: 11.205

3. Sodium pump isozymes are differentially expressed in electrically dissimilar regions of colonic circular smooth muscle.

Authors: E P Burke; K M Sanders; B Horowitz
Journal: Proc Natl Acad Sci U S A Date: 1991-03-15 Impact factor: 11.205

10. Effects of maternal diabetes on fetal rat lung ion transport. Contribution of alveolar and bronchiolar epithelial cells to Na+,K(+)-ATPase expression.

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