Christopher Ludtka1,2, Stefan Schwan3,4, Andrea Friedmann1,5, Walther Brehm5,6, Ingo Wiesner7, Felix Goehre7,8. 1. Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Halle (Saale), Germany. 2. Department of Chemical and Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, USA. 3. Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Halle (Saale), Germany. stefan.schwan@imws.fraunhofer.de. 4. Translational Center for Regenerative Medicine, University of Leipzig, Leipzig, Germany. stefan.schwan@imws.fraunhofer.de. 5. Translational Center for Regenerative Medicine, University of Leipzig, Leipzig, Germany. 6. Department of Veterinary Medicine, University of Leipzig, Leipzig, Germany. 7. Department of General Surgery, BG Bergmannstrost Clinic, Halle, Germany. 8. Department of Neurosurgery, University of Helsinki, Helsinki, Finland.
Abstract
PURPOSE: The primary goal of this study is to clearly define and evaluate new intervertebral disc height parameters in analysing the morphological pathology of disc degeneration for application in damage model and regeneration therapy development, as well as applying traditional variables to 3-D characterization methods. METHODS: A posterolateral surgical approach was used to induce disc degeneration in an ovine model. At 12-months post-operation, sheep vertebral segments were removed and characterized using micro-CT to evaluate disc height parameters in regard to injury localization. RESULTS: Statistically significant differences between the disc height loss of the left and right side of the disc, consistent with the lateral surgical approach used were seen using the modified average disc height method by Dabbs et al. However, convexity index and the newly proposed Cross Tilt Index did not conclusively demonstrate a difference. CONCLUSION: Two-dimensional morphological evaluations can be applied in 3-D to provide a more complete picture of disc height loss for injury models. New 3-D parameters that are tailored to the type of surgical approach used should be investigated, with the 9-point system described herein providing a useful basis for derived values. Additionally, the surgical approach chosen when artificially injuring the disc can result in asymmetrical degeneration, as indicated by uneven disc height loss.
PURPOSE: The primary goal of this study is to clearly define and evaluate new intervertebral disc height parameters in analysing the morphological pathology of disc degeneration for application in damage model and regeneration therapy development, as well as applying traditional variables to 3-D characterization methods. METHODS: A posterolateral surgical approach was used to induce disc degeneration in an ovine model. At 12-months post-operation, sheep vertebral segments were removed and characterized using micro-CT to evaluate disc height parameters in regard to injury localization. RESULTS: Statistically significant differences between the disc height loss of the left and right side of the disc, consistent with the lateral surgical approach used were seen using the modified average disc height method by Dabbs et al. However, convexity index and the newly proposed Cross Tilt Index did not conclusively demonstrate a difference. CONCLUSION: Two-dimensional morphological evaluations can be applied in 3-D to provide a more complete picture of disc height loss for injury models. New 3-D parameters that are tailored to the type of surgical approach used should be investigated, with the 9-point system described herein providing a useful basis for derived values. Additionally, the surgical approach chosen when artificially injuring the disc can result in asymmetrical degeneration, as indicated by uneven disc height loss.
Authors: Peter Ghosh; Robert Moore; Barrie Vernon-Roberts; Tony Goldschlager; Diane Pascoe; Andrew Zannettino; Stan Gronthos; Silviu Itescu Journal: J Neurosurg Spine Date: 2012-03-09
Authors: T Maerz; M D Newton; K Kristof; O Motovylyak; J S Fischgrund; D K Park; K C Baker Journal: Osteoarthritis Cartilage Date: 2014-08-20 Impact factor: 6.576
Authors: Egon Perilli; Ian H Parkinson; Le-Hoa Truong; Kuan C Chong; Nicola L Fazzalari; Orso L Osti Journal: Eur Spine J Date: 2014-07-26 Impact factor: 3.134
Authors: John M Peloquin; Jonathon H Yoder; Nathan T Jacobs; Sung M Moon; Alexander C Wright; Edward J Vresilovic; Dawn M Elliott Journal: J Biomech Date: 2014-04-18 Impact factor: 2.712
Authors: Zhen Wang; Carman M Perez-Terzic; Jay Smith; William D Mauck; Randy A Shelerud; Timothy P Maus; Tai-Hua Yang; Mohammad Hassan Murad; Shanmiao Gou; Marisa J Terry; Jason P Dauffenbach; Mathew J Pingree; Jason S Eldrige; Khaled Mohammed; Khalid Benkhadra; Andre J van Wijnen; Wenchun Qu Journal: Gene Date: 2015-03-19 Impact factor: 3.688
Authors: Andrea Friedmann; Andre Baertel; Christine Schmitt; Christopher Ludtka; Javorina Milosevic; Hans-Joerg Meisel; Felix Goehre; Stefan Schwan Journal: Int J Mol Sci Date: 2021-04-19 Impact factor: 5.923
Authors: Naomi N Lee; Elias Salzer; Frances C Bach; Andres F Bonilla; James L Cook; Zulma Gazit; Sibylle Grad; Keita Ito; Lachlan J Smith; Andrea Vernengo; Hans-Joachim Wilke; Julie B Engiles; Marianna A Tryfonidou Journal: JOR Spine Date: 2021-06-14