Literature DB >> 28286807

Protein Synthesis Rate Assessment by Fluorescence Recovery after Photobleaching (FRAP).

Nikos Kourtis1, Nektarios Tavernarakis2.   

Abstract

Currently available biochemical methods cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. Here, we describe a non-invasive method for monitoring protein synthesis in single cells or tissues with intrinsically different translation rates, in live Caenorhabditis elegans animals.

Entities:  

Keywords:  Caenorhabditis elegans; FRAP; Messenger RNA; Protein synthesis; Protein translation

Year:  2017        PMID： 28286807      PMCID： PMC5344182          DOI： 10.21769/BioProtoc.2156

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  7 in total

1.  eIF4E function in somatic cells modulates ageing in Caenorhabditis elegans.

Authors:  Popi Syntichaki; Kostoula Troulinaki; Nektarios Tavernarakis
Journal:  Nature       Date:  2007-02-04       Impact factor: 49.962

2.  Genetic, behavioral and environmental determinants of male longevity in Caenorhabditis elegans.

Authors:  D Gems; D L Riddle
Journal:  Genetics       Date:  2000-04       Impact factor: 4.562

Review 3.  Measurement of human tissue protein synthesis: an optimal approach.

Authors:  M J Rennie; K Smith; P W Watt
Journal:  Am J Physiol       Date:  1994-03

Review 4.  Basic culture methods.

Authors:  J A Lewis; J T Fleming
Journal:  Methods Cell Biol       Date:  1995       Impact factor: 1.441

Review 5.  Lost in translation: dysregulation of cap-dependent translation and cancer.

Authors:  Mary-Ann Bjornsti; Peter J Houghton
Journal:  Cancer Cell       Date:  2004-06       Impact factor: 31.743

6.  Genetic analysis of tissue aging in Caenorhabditis elegans: a role for heat-shock factor and bacterial proliferation.

Authors:  Delia Garigan; Ao-Lin Hsu; Andrew G Fraser; Ravi S Kamath; Julie Ahringer; Cynthia Kenyon
Journal:  Genetics       Date:  2002-07       Impact factor: 4.562

7.  Transgenesis in Caenorhabditis elegans.

Authors:  Matthias Rieckher; Nikos Kourtis; Angela Pasparaki; Nektarios Tavernarakis
Journal:  Methods Mol Biol       Date:  2009
  7 in total
  2 in total

1.  Abnormal RNA stability in amyotrophic lateral sclerosis.

Authors:  E M Tank; C Figueroa-Romero; L M Hinder; K Bedi; H C Archbold; X Li; K Weskamp; N Safren; X Paez-Colasante; C Pacut; S Thumma; M T Paulsen; K Guo; J Hur; M Ljungman; E L Feldman; S J Barmada
Journal:  Nat Commun       Date:  2018-07-20       Impact factor: 14.919

2.  Maintenance of Proteostasis by P Body-Mediated Regulation of eIF4E Availability during Aging in Caenorhabditis elegans.

Authors:  Matthias Rieckher; Maria Markaki; Andrea Princz; Björn Schumacher; Nektarios Tavernarakis
Journal:  Cell Rep       Date:  2018-10-02       Impact factor: 9.423
  2 in total

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