Denia M Piña-Vázquez1, Zyanya Mayoral-Peña1, Maricela Gómez-Sánchez2, Luis A Salazar-Olivo3, Fausto Arellano-Carbajal4. 1. Universidad Autónoma de Querétaro, Facultad de Ciencias Naturales, Av. de las Ciencias S/N, Querétaro, Mexico. 2. Universidad Autónoma de Querétaro, Facultad de Ciencias Naturales, Av. de las Ciencias S/N, Querétaro, Mexico. Electronic address: gomezs@uaq.mx. 3. IPICYT, División de Biología Molecular, Camino a la Presa San José 2055, San Luis Potosí 76216, Mexico. Electronic address: olivo@ipicyt.edu.mx. 4. Universidad Autónoma de Querétaro, Facultad de Ciencias Naturales, Av. de las Ciencias S/N, Querétaro, Mexico. Electronic address: fausto.arellano@uaq.mx.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Psidium guajava and Tagetes erecta have been used traditionally to treat gastrointestinal parasites, but their active metabolites and mechanisms of action remain largely unknown. AIM OF THE STUDY: To evaluate the anthelmintic potential of Psidium guajava and Tagetes erecta extracts on Levamisole-sensitive and Levamisole-resistant strains of the model nematode Caenorhabditis elegans. MATERIALS AND METHODS: Aqueous extracts of Psidium guajava (PGE) and Tagetes erecta (TEE) were assayed on locomotion and egg-laying behaviors of the wild-type (N2) and Levamisole-resistant (CB193) strains of Caenorhabditis elegans. RESULTS: Both extracts paralyzed wild-type and Levamisole-resistant nematodes in a dose-dependent manner. In wild-type worms, TEE 25mg/mL induced a 75% paralysis after 8h of treatment and PGE 25mg/mL induced a 100% paralysis after 4h of treatment. PGE exerted a similar paralyzing effect on N2 wild-type and CB193 Levamisole-resistant worms, while TEE only partially paralyzed CB193 worms. TEE 25mg/mL decreased N2 egg-laying by 65% with respect to the untreated control, while PGE did it by 40%. CONCLUSIONS: Psidium guajava leaves and Tagetes erecta flower-heads possess hydrosoluble compounds that block the motility of Caenorhabditis elegans by a mechanism different to that of the anthelmintic drug Levamisole. Effects are also observable on oviposition, which was diminished in the wild-type worms. The strong anthelmintic effects in crude extracts of these plants warrants future work to identify their active compounds and to elucidate their molecular mechanisms of action.
ETHNOPHARMACOLOGICAL RELEVANCE: Psidium guajava and Tagetes erecta have been used traditionally to treat gastrointestinal parasites, but their active metabolites and mechanisms of action remain largely unknown. AIM OF THE STUDY: To evaluate the anthelmintic potential of Psidium guajava and Tagetes erecta extracts on Levamisole-sensitive and Levamisole-resistant strains of the model nematode Caenorhabditis elegans. MATERIALS AND METHODS: Aqueous extracts of Psidium guajava (PGE) and Tagetes erecta (TEE) were assayed on locomotion and egg-laying behaviors of the wild-type (N2) and Levamisole-resistant (CB193) strains of Caenorhabditis elegans. RESULTS: Both extracts paralyzed wild-type and Levamisole-resistant nematodes in a dose-dependent manner. In wild-type worms, TEE 25mg/mL induced a 75% paralysis after 8h of treatment and PGE 25mg/mL induced a 100% paralysis after 4h of treatment. PGE exerted a similar paralyzing effect on N2 wild-type and CB193 Levamisole-resistant worms, while TEE only partially paralyzed CB193 worms. TEE 25mg/mL decreased N2 egg-laying by 65% with respect to the untreated control, while PGE did it by 40%. CONCLUSIONS:Psidium guajava leaves and Tagetes erecta flower-heads possess hydrosoluble compounds that block the motility of Caenorhabditis elegans by a mechanism different to that of the anthelmintic drug Levamisole. Effects are also observable on oviposition, which was diminished in the wild-type worms. The strong anthelmintic effects in crude extracts of these plants warrants future work to identify their active compounds and to elucidate their molecular mechanisms of action.
Authors: Carlos A Vásquez-Londoño; Luisa F Cubillos-Cuadrado; Andrea C Forero-Ozer; Paola A Escobar-Espinosa; David O Cubillos-López; Daniel F Castaño-Betancur Journal: Adv Exp Med Biol Date: 2021 Impact factor: 2.622
Authors: Gerald Zirintunda; Savino Biryomumaisho; Keneth Iceland Kasozi; Gaber El-Saber Batiha; John Kateregga; Patrick Vudriko; Sarah Nalule; Deogracious Olila; Mariam Kajoba; Kevin Matama; Mercy Rukundo Kwizera; Mohammed M Ghoneim; Mahmoud Abdelhamid; Sameh S Zaghlool; Sultan Alshehri; Mohamed A Abdelgawad; James Acai-Okwee Journal: Front Pharmacol Date: 2022-02-14 Impact factor: 5.810