Talita Mendes da Silva Ventura1, Luiza de Paula Silva Cassiano2, Cíntia Maria de Souza E Silva3, Even Akemi Taira4, Aline de Lima Leite5, Daniela Rios6, Marília Afonso Rabelo Buzalaf7. 1. Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: talitaventura@usp.br. 2. Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: luizacassiano@hotmail.com. 3. Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: cintiaours@gmail.com. 4. Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: even.akemi@gmail.com. 5. Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: a.lima82@yahoo.com.br. 6. Department of Pediatric Dentistry, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: danirios@usp.br. 7. Department of Biological Science, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: mbuzalaf@fob.usp.br.
Abstract
OBJECTIVE: This study evaluated the variation in the protein profile of the acquired enamel pellicle (AEP) formed in vivo according to its location in the dental arches. DESIGN: The AEP was formed for 120min in 9 volunteers. Pellicle formed at upper+lower anterior facial (ULAFa; teeth 13-23 and 33-43), upper anterior palatal (UAPa; teeth 13-23), lower anterior lingual (LALi; teeth 33-43), upper+lower posterior facial (ULPFa; teeth 14-17 24-27, 34-37 and 44-47), upper posterior palatal (UPPa; teeth 14-17 and 24-27) and lower posterior lingual (LPLi; teeth 34-37 and 44-47) regions were collected separately and processed for analysis by label-free LC-ESI-MS/MS. RESULTS: Three-hundred sixty three proteins were identified in total, twenty-five being common to all the locations, such as Protein S100-A8, Lysozyme C, Lactoferrin, Statherin, Ig alpha-2, ALB protein, Myeloperoxidase and SMR3B. Many proteins were found exclusively in the AEP collected from one of the regions (46-UAPa, 33-LALi, 59-ULAFa, 31-ULPFa, 44-LPLi and 39-UPPa). CONCLUSIONS: The protein composition of the AEP varied according to its location in the dental arches. These results provide important insights for understanding the differential protective roles of the AEP as a function of its location in the dental arches.
OBJECTIVE: This study evaluated the variation in the protein profile of the acquired enamel pellicle (AEP) formed in vivo according to its location in the dental arches. DESIGN: The AEP was formed for 120min in 9 volunteers. Pellicle formed at upper+lower anterior facial (ULAFa; teeth 13-23 and 33-43), upper anterior palatal (UAPa; teeth 13-23), lower anterior lingual (LALi; teeth 33-43), upper+lower posterior facial (ULPFa; teeth 14-17 24-27, 34-37 and 44-47), upper posterior palatal (UPPa; teeth 14-17 and 24-27) and lower posterior lingual (LPLi; teeth 34-37 and 44-47) regions were collected separately and processed for analysis by label-free LC-ESI-MS/MS. RESULTS: Three-hundred sixty three proteins were identified in total, twenty-five being common to all the locations, such as Protein S100-A8, Lysozyme C, Lactoferrin, Statherin, Ig alpha-2, ALB protein, Myeloperoxidase and SMR3B. Many proteins were found exclusively in the AEP collected from one of the regions (46-UAPa, 33-LALi, 59-ULAFa, 31-ULPFa, 44-LPLi and 39-UPPa). CONCLUSIONS: The protein composition of the AEP varied according to its location in the dental arches. These results provide important insights for understanding the differential protective roles of the AEP as a function of its location in the dental arches.
Authors: Tarsila M Camargo; Rafael N Stipp; Lívia A Alves; Erika N Harth-Chu; José F Höfling; Renata O Mattos-Graner Journal: Infect Immun Date: 2018-03-22 Impact factor: 3.441
Authors: Galina Laputková; Vladimíra Schwartzová; Juraj Bánovčin; Michal Alexovič; Ján Sabo Journal: Open Life Sci Date: 2018-05-18 Impact factor: 0.938