Caroline Moreira Auersvald1, Felipe Rychuv Santos1, Mayara Mytie Nakano1, Graziela Bianchi Leoni2, Manoel Damião de Sousa Neto2, Rafaela Scariot1, Allan Fernando Giovanini1, Tatiana Miranda Deliberador3. 1. Graduate Program in Dentistry, Universidade Positivo, R. Prof. Pedro Viriato Parigot de Souza, 5300 - Cidade Industrial, Curitiba, PR, 81280-330, Brazil. 2. Departamento de Odontologia Restauradora, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/no - Campus da USP Ribeirão Preto, SP, CEP 14040-904, Brazil. 3. Graduate Program in Dentistry, Universidade Positivo, R. Prof. Pedro Viriato Parigot de Souza, 5300 - Cidade Industrial, Curitiba, PR, 81280-330, Brazil. Electronic address: tdeliberador@gmail.com.
Abstract
OBJECTIVE: To evaluate the effect of a single-dose local administration of PTH on bone healing in rat calvarial bone defects by means of micro-computed tomography, histological and histomorphometric analysis. DESIGN: Critical-size cranial osteotomy defects were created in 42 male rats. The animals were randomly divided into 3 groups. In the C Group, the bone defect was only filled with a blood clot. In the S Group, it was filled with a collagen sponge and covered with bovine cortical membrane. In the PTH Group, the defect was filled with a collagen sponge soaked with PTH and covered with bovine cortical membrane. The groups were further split in two for euthanasia 15 and 60days post-surgery. Data was statistically analyzed with t-tests for independent samples or the nonparametric Mann-Whitney test when applicable. Intragroup comparisons were analyzed with paired t-tests (p<0.05). RESULTS: Micro-CT analysis results did not demonstrate statistically significant intergroup differences. At 15days post-surgery, the histomorphometric analysis showed that the PTH Group exhibited a significantly higher percentage of bone formation compared with the S Group. At 60days post-surgery, a higher percentage of new bone was observed in the PTH group. CONCLUSION: The results suggest that the local administration of PTH encouraged the bone healing in critical-size calvarial defects in rats.
OBJECTIVE: To evaluate the effect of a single-dose local administration of PTH on bone healing in rat calvarial bone defects by means of micro-computed tomography, histological and histomorphometric analysis. DESIGN: Critical-size cranial osteotomy defects were created in 42 male rats. The animals were randomly divided into 3 groups. In the C Group, the bone defect was only filled with a blood clot. In the S Group, it was filled with a collagen sponge and covered with bovine cortical membrane. In the PTH Group, the defect was filled with a collagen sponge soaked with PTH and covered with bovine cortical membrane. The groups were further split in two for euthanasia 15 and 60days post-surgery. Data was statistically analyzed with t-tests for independent samples or the nonparametric Mann-Whitney test when applicable. Intragroup comparisons were analyzed with paired t-tests (p<0.05). RESULTS: Micro-CT analysis results did not demonstrate statistically significant intergroup differences. At 15days post-surgery, the histomorphometric analysis showed that the PTH Group exhibited a significantly higher percentage of bone formation compared with the S Group. At 60days post-surgery, a higher percentage of new bone was observed in the PTH group. CONCLUSION: The results suggest that the local administration of PTH encouraged the bone healing in critical-size calvarial defects in rats.