Saman Heydari1, Saeed Ghanbarzadeh2, Behzad Anoush3, Mohammadreza Ranjkesh3, Yousef Javadzadeh4, Maryam Kouhsoltani5, Hamed Hamishehkar6. 1. a Institute for Stem Cell and Regenerative Medicine and Students' Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences , Tabriz , Iran. 2. b Zanjan Pharmaceutical Nanotechnology Research Center and Department of Pharmaceutics, Faculty of Pharmacy , Zanjan University of Medical Sciences , Zanjan , Iran. 3. c Dermatology & Dermopharmacy Research Team and Department of Dermatology, Sina Hospital , Tabriz University of Medical Sciences , Tabriz , Iran. 4. d Biotechnology Research Center and Faculty of Pharmacy , Tabriz University of Medical Sciences , Tabriz , Iran. 5. e Department of Oral & Maxillofacial Pathology, Faculty of Dentistry , Tabriz University of Medical Sciences , Tabriz , Iran. 6. f Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Abstract
BACKGROUND: Free radical scavengers and antioxidants, with the main focus on enhanced targeting to the skin layers, can provide protection against skin ageing. OBJECTIVE: The aim of the present study was to prepare nanoethosomal formulation of gammaoryzanol (GO), a water insoluble antioxidant, for its dermal delivery to prevent skin aging. METHODS: Nanoethosomal formulation was prepared by a modified ethanol injection method and characterized by using laser light scattering, scanning electronic microscope (SEM) and X-ray diffraction (XRD) techniques. The effects of formulation parameters on nanoparticle size, encapsulation efficiency percent (EE%) and loading capacity percent (LC%) were investigated. Antioxidant activity of GO-loaded formulation was investigated in vitro using normal African green monkey kidney fibroblast cells (Vero). The effect of control and GO-loaded nanoethosomal formulation on superoxide dismutase (SOD) and malondialdehyde (MDA) content of rat skin was also probed. Furthermore, the effect of GO-loaded nanoethosomes on skin wrinkle improvement was studied by dermoscopic and histological examination on healthy humans and UV-irradiated rats, respectively. RESULTS: The optimized nanoethosomal formulation showed promising characteristics including narrow size distribution 0.17 ± 0.02, mean diameter of 98.9 ± 0.05 nm, EE% of 97.12 ± 3.62%, LC% of 13.87 ± 1.36% and zeta potential value of -15.1 ± 0.9 mV. The XRD results confirmed uniform drug dispersion in the nanoethosomes structure. In vitro and in vivo antioxidant studies confirmed the superior antioxidant effect of GO-loaded nanoethosomal formulation compared with control groups (blank nanoethosomes and GO suspension). CONCLUSIONS: Nanoethosomes was a promising carrier for dermal delivery of GO and consequently had superior anti-aging effect.
BACKGROUND:Free radical scavengers and antioxidants, with the main focus on enhanced targeting to the skin layers, can provide protection against skin ageing. OBJECTIVE: The aim of the present study was to prepare nanoethosomal formulation of gammaoryzanol (GO), a water insoluble antioxidant, for its dermal delivery to prevent skin aging. METHODS: Nanoethosomal formulation was prepared by a modified ethanol injection method and characterized by using laser light scattering, scanning electronic microscope (SEM) and X-ray diffraction (XRD) techniques. The effects of formulation parameters on nanoparticle size, encapsulation efficiency percent (EE%) and loading capacity percent (LC%) were investigated. Antioxidant activity of GO-loaded formulation was investigated in vitro using normal African green monkey kidney fibroblast cells (Vero). The effect of control and GO-loaded nanoethosomal formulation on superoxide dismutase (SOD) and malondialdehyde (MDA) content of rat skin was also probed. Furthermore, the effect of GO-loaded nanoethosomes on skin wrinkle improvement was studied by dermoscopic and histological examination on healthy humans and UV-irradiated rats, respectively. RESULTS: The optimized nanoethosomal formulation showed promising characteristics including narrow size distribution 0.17 ± 0.02, mean diameter of 98.9 ± 0.05 nm, EE% of 97.12 ± 3.62%, LC% of 13.87 ± 1.36% and zeta potential value of -15.1 ± 0.9 mV. The XRD results confirmed uniform drug dispersion in the nanoethosomes structure. In vitro and in vivo antioxidant studies confirmed the superior antioxidant effect of GO-loaded nanoethosomal formulation compared with control groups (blank nanoethosomes and GO suspension). CONCLUSIONS: Nanoethosomes was a promising carrier for dermal delivery of GO and consequently had superior anti-aging effect.
Entities:
Keywords:
Gammaoryzanol; dermal drug delivery; nanoethosomes; skin aging; wrinkle
Authors: K M Aguilar-Pérez; J I Avilés-Castrillo; Dora I Medina; Roberto Parra-Saldivar; Hafiz M N Iqbal Journal: Front Bioeng Biotechnol Date: 2020-12-15