Mathavi Sahadevan1, Oukseub Lee1, Miguel Muzzio2, Belinda Phan1, Lisa Jacobs3, Nagi Khouri3, Jun Wang1, Hong Hu1, Vered Stearns3, Robert T Chatterton4,5. 1. a Department of Surgery , Northwestern University Feinberg School of Medicine , Chicago , IL , USA. 2. b Analytical Chemistry Division , IIT Research Institute , Chicago , IL , USA. 3. c Johns Hopkins School of Medicine , Breast Cancer Program, Kimmel Cancer Center , Baltimore , MD , USA. 4. d Departments of Obstetrics/Gynaecology, Physiology, and Pathology , Northwestern University Feinberg School of Medicine , Chicago , IL , USA. 5. e Robert H. Lurie Comprehensive Cancer Center of Northwestern University , Chicago , IL , USA.
Abstract
CONTEXT: Clinical study of breast cancer patients in Chicago, IL, USA. OBJECTIVE: Ascertain the utility of measurements of single-strand breaks (SSB) in DNA for assessment of breast cancer risk. METHODS: Fine-needle aspirates of the breast, SSB by nick translation, percent breast density (PBD), Gail model risk, cumulative methylation index (CMI), enzymes of DNA repair and tissue antioxidants. RESULTS: DNA repair enzymes and 4-hydroxyestradiol were negatively associated with SSB; CMI and PBD were positively associated. CONCLUSIONS: Quantitative measurement of SSBs by this procedure indicates the relative number of SSBs and is related to promoter methylation, antioxidant availability and percent breast density.
CONTEXT: Clinical study of breast cancerpatients in Chicago, IL, USA. OBJECTIVE: Ascertain the utility of measurements of single-strand breaks (SSB) in DNA for assessment of breast cancer risk. METHODS: Fine-needle aspirates of the breast, SSB by nick translation, percent breast density (PBD), Gail model risk, cumulative methylation index (CMI), enzymes of DNA repair and tissue antioxidants. RESULTS: DNA repair enzymes and 4-hydroxyestradiol were negatively associated with SSB; CMI and PBD were positively associated. CONCLUSIONS: Quantitative measurement of SSBs by this procedure indicates the relative number of SSBs and is related to promoter methylation, antioxidant availability and percent breast density.
Entities:
Keywords:
Breast cancer; DNA methylation; breast cancer risk; single-strand breaks in DNA; tissue oestrogens