Literature DB >> 2827613

Expression of an early Epstein-Barr virus antigen (EA-D) in E. coli. Brief report.

D Roeckel1, H Boos, N Mueller-Lantzsch.   

Abstract

The 1.34 kb BcII-BgIII-fragment of the BamHI-M region of Epstein-Barr virus genome, comprising the complete BMRF1 open reading frame, was cloned into the tryptophan regulated E. coli expression vector pATH1. The resulting fusion protein, having a molecular weight of 80 kd, is recognized not only by anti-early antigen (EA)-positive human sera but also by the monoclonal antibody R3 directed against the diffuse component of EA (EA-D). A possible use for this fusion protein as an indicator protein in diagnosis of IgA antibodies against EA-D is presented.

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Year:  1987        PMID： 2827613     DOI： 10.1007/BF01314434

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  28 in total

1.  Immunofluorescence in cells derived from Burkitt's lymphoma.

Authors:  G Henle; W Henle
Journal:  J Bacteriol       Date:  1966-03       Impact factor: 3.490

2.  Epstein-Barr virus (P3HR-1) defective DNA codes for components of both the early antigen and viral capsid antigen complexes.

Authors:  M S Cho; L Gissmann; S D Hayward
Journal:  Virology       Date:  1984-08       Impact factor: 3.616

3.  Enzyme-linked immunosorbent assay for the detection of Epstein-Barr virus-induced antigens and antibodies.

Authors:  L Sternås; J Luka; B Kallin; A Rosén; W Henle; G Henle; G Klein
Journal:  J Immunol Methods       Date:  1983-10-14       Impact factor: 2.303

4.  Enzyme-linked immunosorbent assay of antibodies to Epstein-Barr virus nuclear and early antigens in patients with infectious mononucleosis and nasopharyngeal carcinoma.

Authors:  J Halprin; A L Scott; L Jacobson; P H Levine; J H Ho; J C Niederman; S D Hayward; G Milman
Journal:  Ann Intern Med       Date:  1986-03       Impact factor: 25.391

5.  Identification and mapping of Epstein-Barr virus early antigens and demonstration of a viral gene activator that functions in trans.

Authors:  K M Wong; A J Levine
Journal:  J Virol       Date:  1986-10       Impact factor: 5.103

6.  Immunobiochemical characterization with monoclonal antibodies of Epstein-Barr virus-associated early antigens in chemically induced cells.

Authors:  A L Epstein
Journal:  J Virol       Date:  1984-05       Impact factor: 5.103

7.  Expression of a nuclear and a cytoplasmic Epstein-Barr virus early antigen after DNA transfer: cooperation of two distant parts of the genome for expression of the cytoplasmic antigen.

Authors:  K Takaki; A Polack; G W Bornkamm
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

8.  Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli.

Authors:  K R Spindler; D S Rosser; A J Berk
Journal:  J Virol       Date:  1984-01       Impact factor: 5.103

9.  Epstein-Barr virus-induced proteins. IV. Characterization of an EBV-associated phosphopolypeptide.

Authors:  N Mueller-Lantzsch; N Yamamoto
Journal:  J Gen Virol       Date:  1981-08       Impact factor: 3.891

10.  Differential reactivity of human serums with early antigens induced by Epstein-Barr virus.

Authors:  W Henle; G Henle; B A Zajac; G Pearson; R Waubke; M Scriba
Journal:  Science       Date:  1970-07-10       Impact factor: 47.728
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  1 in total

Review 1.  Mapping of viral epitopes with prokaryotic expression products.

Authors:  J A Lenstra; J G Kusters; B A van der Zeijst
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574
  1 in total

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