Literature DB >> 2827538

High-performance liquid chromatographic analysis of oligodeoxyribonucleotide base composition.

J S Eadie1, L J McBride, J W Efcavitch, L B Hoff, R Cathcart.   

Abstract

A significantly improved method for base composition analysis of synthetic oligodeoxyribonucleotides is presented. This highly accurate and sensitive method used enzymatic digestion followed by high-resolution HPLC of the nucleosides to determine the empirical base composition of the parent compound. The enzymatic digestion reaction is quantitative and is not blocked by modified bases, thus allowing the degree of base deprotection and chemical modification to be assessed. Digestion data are presented for oligodeoxyribonucleotides which range from 18 to 150 bases in length with excellent agreement of experimental and theoretical composition. The method is also applicable to high-molecular-weight genomic DNA.

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Year:  1987        PMID： 2827538     DOI： 10.1016/0003-2697(87)90294-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  22 in total

1.  Boron trifluoride-methanol complex as a non-depurinating detritylating agent in DNA synthesis.

Authors:  M J Mitchell; W Hirschowitz; F Rastinejad; P Lu
Journal:  Nucleic Acids Res       Date:  1990-09-11       Impact factor: 16.971

2.  Elucidation of the sequence-specific third-strand recognition of four Watson-Crick base pairs in a pyrimidine triple-helix motif: T.AT, C.GC, T.CG, and G.TA.

Authors:  K Yoon; C A Hobbs; J Koch; M Sardaro; R Kutny; A L Weis
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205

3.  Use of synthetic oligoribonucleotides to probe RNA-protein interactions in the MS2 translational operator complex.

Authors:  S J Talbot; S Goodman; S R Bates; C W Fishwick; P G Stockley
Journal:  Nucleic Acids Res       Date:  1990-06-25       Impact factor: 16.971

4.  Synthesis of oligonucleotides containing 2'-deoxy-6-thioguanosine at a predetermined site.

Authors:  M S Christopherson; A D Broom
Journal:  Nucleic Acids Res       Date:  1991-10-25       Impact factor: 16.971

5.  Oligonucleotide-mediated triple helix formation using an N3-protonated deoxycytidine analog exhibiting pH-independent binding within the physiological range.

Authors:  S H Krawczyk; J F Milligan; S Wadwani; C Moulds; B C Froehler; M D Matteucci
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205

6.  Synthesis and restriction enzyme analysis of oligodeoxyribonucleotides containing the anti-cancer drug 2',2'-difluoro-2'-deoxycytidine.

Authors:  F C Richardson; K K Richardson; J S Kroin; L W Hertel
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

7.  The synthesis and stability of oligodeoxyribonucleotides containing the deoxyadenosine mimic 1-(2'-deoxy-beta-D-ribofuranosyl)imidazole-4-carboxamide.

Authors:  W T Johnson; P Zhang; D E Bergstrom
Journal:  Nucleic Acids Res       Date:  1997-02-01       Impact factor: 16.971

8.  Structure of a small RNA hairpin.

Authors:  P W Davis; W Thurmes; I Tinoco
Journal:  Nucleic Acids Res       Date:  1993-02-11       Impact factor: 16.971

9.  Synthesis of oligodeoxynucleotides containing 2-thiopyrimidine residues--a new protection scheme.

Authors:  R G Kuimelis; K P Nambiar
Journal:  Nucleic Acids Res       Date:  1994-04-25       Impact factor: 16.971

10.  Large scale, liquid phase synthesis of oligonucleotides by the phosphoramidite approach.

Authors:  G M Bonora; G Biancotto; M Maffini; C L Scremin
Journal:  Nucleic Acids Res       Date:  1993-03-11       Impact factor: 16.971
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