| Literature DB >> 28270968 |
Dongli Xu1, Weibin Zhou2, Leilei Peng3.
Abstract
Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.Entities:
Keywords: (110.0110) Imaging systems; (170.2520) Fluorescence microscopy; (170.6280) Spectroscopy, fluorescence and luminescence; (180.6900) Three-dimensional microscopy
Year: 2017 PMID： 28270968 PMCID： PMC5330577 DOI： 10.1364/BOE.8.000570
Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732