Jianliang Deng1,1, Wenjiao Chen1,1, Yuan Du2,1, Weiming Wang1, Guoqiang Zhang1, Yuehua Tang1, Zhangjun Qian1, Ping Xu1, Zhihong Cao3, Yan Zhou1. 1. Department of Oncology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China. 2. Department of Pathology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China. 3. Department of Radiology, the Affiliated Yixing Hospital of Jiangsu University, Wuxi, Jiangsu 214200, China.
Abstract
BACKGROUND: Cullin1 and MMP-2 have been identified as important markers in various cancers, but their roles in colorectal cancer (CRC) have remained to be discovered. The aim of this study was to investigate the expression pattern and significance of Cullin1 and MMP-2 in CRC. METHODS: A total of 470 CRC patients were enrolled. Archival paraffin-embedded CRC tissue samples were used to generate tissue microarray blocks, which were immunohistochemically stained for Cullin1 and MMP-2. Prognostic and predictive role of Cullin1 and MMP-2 expression was evaluated by univariate and multivariate analysis, respectively. Values of p < 0.05 were considered statistically significant. RESULTS: Cullin1 and MMP-2 protein levels were significantly upregulated in CRC tissues compared with adjacent noncancerous tissues. High tumoral Cullin1 or MMP-2 expression significantly correlated with shorter overall survival (OS), as well as with clinicopathologic characteristics in patients. Multivariate regression analysis showed that high Cullin1 and MMP-2 expressions, separately and together, were independent negative markers of OS. CONCLUSION: Cullin1 and MMP-2 expressions could be novel diagnostic and prognostic markers for CRC patients.
BACKGROUND:Cullin1 and MMP-2 have been identified as important markers in various cancers, but their roles in colorectal cancer (CRC) have remained to be discovered. The aim of this study was to investigate the expression pattern and significance of Cullin1 and MMP-2 in CRC. METHODS: A total of 470 CRC patients were enrolled. Archival paraffin-embedded CRC tissue samples were used to generate tissue microarray blocks, which were immunohistochemically stained for Cullin1 and MMP-2. Prognostic and predictive role of Cullin1 and MMP-2 expression was evaluated by univariate and multivariate analysis, respectively. Values of p < 0.05 were considered statistically significant. RESULTS:Cullin1 and MMP-2 protein levels were significantly upregulated in CRC tissues compared with adjacent noncancerous tissues. High tumoral Cullin1 or MMP-2 expression significantly correlated with shorter overall survival (OS), as well as with clinicopathologic characteristics in patients. Multivariate regression analysis showed that high Cullin1 and MMP-2 expressions, separately and together, were independent negative markers of OS. CONCLUSION:Cullin1 and MMP-2 expressions could be novel diagnostic and prognostic markers for CRC patients.
Authors: Loganathan Rangasamy; Bruno Di Geronimo; Irene Ortín; Claire Coderch; José María Zapico; Ana Ramos; Beatriz de Pascual-Teresa Journal: Molecules Date: 2019-08-16 Impact factor: 4.411
Authors: Aline Simoneti Fonseca; Anelisa Ramão; Matheus Carvalho Bürger; Jorge Estefano Santana de Souza; Dalila Lucíola Zanette; Greice Andreotti de Molfetta; Luiza Ferreira de Araújo; Rafaela de Barros E Lima Bueno; Graziela Moura Aguiar; Jessica Rodrigues Plaça; Cleidson de Pádua Alves; Anemari Ramos Dinarte Dos Santos; Daniel Onofre Vidal; Gyl Eanes Barros Silva; Rodrigo Alexandre Panepucci; Fernanda Maris Peria; Omar Feres; José Joaquim Ribeiro da Rocha; Marco Antonio Zago; Wilson Araújo Silva Journal: BMC Cancer Date: 2021-03-01 Impact factor: 4.430