Devinder P Singh1, Arvind U Gowda2, Karan Chopra3, Michael Tholen4, Sarah Chang4, Vasilios Mavrophilipos4, Nina Semsarzadeh4, Yvonne Rasko1, Luther Holton Iii1. 1. Division of Plastic Surgery, Anne Arundel Medical Center, Annapolis, MD. 2. Section of Plastic and Reconstructive Surgery, Yale University School of Medicine, New Haven, CT. 3. Division of Plastic and Reconstructive Surgery, University of Maryland School of Medicine, Baltimore, MD; Department of Plastic and Reconstructive Surgery, The Johns Hopkins Hospital, Baltimore, MD. 4. Division of Plastic and Reconstructive Surgery, University of Maryland School of Medicine, Baltimore, MD.
Abstract
OBJECTIVE: This study evaluates the effect of negative pressure wound therapy with antiseptic instillation (NPWTi) in the clearance of infection and biofilm formation in an in vivo model of infected spinal implants compared to traditional treatment modalities. MATERIALS AND METHODS: Five pigs underwent titanium rod implantation of their spinous processes followed by injection of 1 x 106 CFUs/100μL of methicillin-resistant Staphylococcus aureus through the fascia at each site. At 1 week postoperatively, an experimental arm of 3 pigs received NPWTi, and a control arm of 2 pigs received wet-to-dry dressings. The persistence of local infection in the experimental group was compared to the control group using tissue cultures. Biofilm development on spinal implants was evaluated using scanning electron microscopy. RESULTS: Mean bacterial count showed a statistical difference between the experimental and the control groups (P < .05). Scanning electron microscopy revealed the presence of uniform biofilm formation across the surface of control group instrumentation, whereas the experimental group showed interrupted areas between biofilm formations. CONCLUSION: The authors concluded that NPWTi is associated with decreased bacterial load and biofilm formation compared to wet-to-dry dressings in an in vivo porcine model of infected spinal instrumentation.
OBJECTIVE: This study evaluates the effect of negative pressure wound therapy with antiseptic instillation (NPWTi) in the clearance of infection and biofilm formation in an in vivo model of infected spinal implants compared to traditional treatment modalities. MATERIALS AND METHODS: Five pigs underwent titanium rod implantation of their spinous processes followed by injection of 1 x 106 CFUs/100μL of methicillin-resistant Staphylococcus aureus through the fascia at each site. At 1 week postoperatively, an experimental arm of 3 pigs received NPWTi, and a control arm of 2 pigs received wet-to-dry dressings. The persistence of local infection in the experimental group was compared to the control group using tissue cultures. Biofilm development on spinal implants was evaluated using scanning electron microscopy. RESULTS: Mean bacterial count showed a statistical difference between the experimental and the control groups (P < .05). Scanning electron microscopy revealed the presence of uniform biofilm formation across the surface of control group instrumentation, whereas the experimental group showed interrupted areas between biofilm formations. CONCLUSION: The authors concluded that NPWTi is associated with decreased bacterial load and biofilm formation compared to wet-to-dry dressings in an in vivo porcine model of infected spinal instrumentation.
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