Literature DB >> 28267624

A TaqMan-based qRT-PCR assay for Senecavirus A detection in tissue samples of neonatal piglets.

Alais Maria Dall Agnol1, Rodrigo Alejandro Arellano Otonel2, Raquel Arruda Leme1, Amauri Alcindo Alfieri3, Alice Fernandes Alfieri1.   

Abstract

This study describes a sensitive (1.3 × 101 genomic copies/μL) and specific TaqMan-based qRT-PCR assay able to detect and quantify SVA RNA in porcine biological samples. The technique represents an efficient tool for the virus diagnosis and assessment of SVA load in tissues of infected animals and for epidemiological studies.
Copyright © 2017 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Picornavirus infection; Quantitative PCR; Real-time RT-PCR; Seneca valley virus; Swine; Vesicular disease

Mesh：

Year:  2017        PMID： 28267624     DOI： 10.1016/j.mcp.2017.03.002

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  8 in total

1.  The third wave of Seneca Valley virus outbreaks in pig herds in southern Brazil.

Authors:  Marcos V Vieira; Carolina Y Yasumitsu; Alais M Dall Agnol; Raquel A Leme; Alice F Alfieri; Amauri A Alfieri
Journal:  Braz J Microbiol       Date:  2022-05-12       Impact factor: 2.214

2.  Development and evaluation of a nested-PCR assay for Senecavirus A diagnosis.

Authors:  Cesar Feronato; Raquel A Leme; Jaqueline A Diniz; Alais Maria Dall Agnol; Alice F Alfieri; Amauri A Alfieri
Journal:  Trop Anim Health Prod       Date:  2017-09-29       Impact factor: 1.559

3.  Rapid and sensitive detection of Senecavirus A by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick method.

Authors:  Jinhui Li; Weifang Liang; Shuaifei Xu; Jian Shi; Xia Zhou; Bowen Liu; Li Yu; Jingfeng Xiong; Guangbin Si; Dongsheng He
Journal:  PLoS One       Date:  2019-05-02       Impact factor: 3.240

4.  Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs.

Authors:  Ling Zhou; Yuan Sun; Jiao-Ling Wu; Kai-Jie Mai; Gui-Hua Chen; Zi-Xian Wu; Yang Bai; Di Li; Zhi-Hai Zhou; Jian Cheng; Rui-Ting Wu; Xiang-Bin Zhang; Jing-Yun Ma
Journal:  J Virol Methods       Date:  2018-02-07       Impact factor: 2.014

Review 5.  A Review on Pathological and Diagnostic Aspects of Emerging Viruses-Senecavirus A, Torque teno sus virus and Linda Virus-In Swine.

Authors:  Salwa Hawko; Giovanni P Burrai; Marta Polinas; Pier Paolo Angioi; Silvia Dei Giudici; Annalisa Oggiano; Alberto Alberti; Chadi Hosri; Elisabetta Antuofermo
Journal:  Vet Sci       Date:  2022-09-10

Review 6.  Update on Senecavirus Infection in Pigs.

Authors:  Raquel A Leme; Alice F Alfieri; Amauri A Alfieri
Journal:  Viruses       Date:  2017-07-03       Impact factor: 5.048

Review 7.  Review of Seneca Valley Virus: A Call for Increased Surveillance and Research.

Authors:  Xiangle Zhang; Zixiang Zhu; Fan Yang; Weijun Cao; Hong Tian; Keshan Zhang; Haixue Zheng; Xiangtao Liu
Journal:  Front Microbiol       Date:  2018-05-11       Impact factor: 5.640

8.  The development of two field-ready reverse transcription loop-mediated isothermal amplification assays for the rapid detection of Seneca Valley virus 1.

Authors:  Bryony Armson; Charlotte Walsh; Nick Morant; Veronica L Fowler; Nick J Knowles; Duncan Clark
Journal:  Transbound Emerg Dis       Date:  2018-11-15       Impact factor: 5.005
  8 in total

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