Anja K Gruenert1, Katja Rosenbaum2, Gerd Geerling2, Thomas A Fuchsluger1. 1. Department of Ophthalmology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany. 2. Department of Ophthalmology, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.
Abstract
PURPOSE: To evaluate the contamination rate and the corresponding spectrum of microbes and to identify donor risk factors for corneal organ culture contaminations. METHODS: A total of 3306 organ-cultured donor corneas were included in the study. We performed a retrospective database analysis to evaluate donor factors such as gender, age, death-to-explantation interval (DEI), procurement site and cause of death and to determine their influence on donor cornea contaminations. Odds ratios (ORs) were calculated for each factor. RESULTS: The overall contamination rate was 7.8% (n = 259). Younger donor age (OR: 2.2, p = 0.003, chi-squared test), a DEI of more than 24 hr (OR: 1.6, p < 0.001), hospitalization prior to death (OR: 2.2, p < 0.001) and death caused by sepsis (OR: 2.7, p < 0.001) were associated with an increased risk of contamination, whereas donor gender did not have an effect on donor cornea contaminations. The most frequently isolated microbes were Enterococci (19%), Staphylococci (10.8%) and Candida (37.4%). CONCLUSION: This study helps to estimate the contamination risk of a cultured cornea based on specific donor factors. However, donors with risk factors should not be generally excluded from cornea donation. Further studies including antibiograms might clarify whether a change in the antibiotic composition of the culture medium would be useful to deal with the increasing number of multi-resistant microbes.
PURPOSE: To evaluate the contamination rate and the corresponding spectrum of microbes and to identify donor risk factors for corneal organ culture contaminations. METHODS: A total of 3306 organ-cultured donor corneas were included in the study. We performed a retrospective database analysis to evaluate donor factors such as gender, age, death-to-explantation interval (DEI), procurement site and cause of death and to determine their influence on donor cornea contaminations. Odds ratios (ORs) were calculated for each factor. RESULTS: The overall contamination rate was 7.8% (n = 259). Younger donor age (OR: 2.2, p = 0.003, chi-squared test), a DEI of more than 24 hr (OR: 1.6, p < 0.001), hospitalization prior to death (OR: 2.2, p < 0.001) and death caused by sepsis (OR: 2.7, p < 0.001) were associated with an increased risk of contamination, whereas donor gender did not have an effect on donor cornea contaminations. The most frequently isolated microbes were Enterococci (19%), Staphylococci (10.8%) and Candida (37.4%). CONCLUSION: This study helps to estimate the contamination risk of a cultured cornea based on specific donor factors. However, donors with risk factors should not be generally excluded from cornea donation. Further studies including antibiograms might clarify whether a change in the antibiotic composition of the culture medium would be useful to deal with the increasing number of multi-resistant microbes.
Authors: Thatiane Lima Sampaio; Isabela Pereira Rodrigues; Micheline Borges Lucas Cresta; Anna Cláudia de Oliveira Queiroz; Thayssa Neiva da Fonseca Victer; Daniela Ferreira Salomão Pontes; Wildo Navegantes de Araújo; Sônia Nair Báo; Maria Regina Catai Chalita Journal: Cell Tissue Bank Date: 2020-11-09 Impact factor: 1.522
Authors: Tobias Röck; Johanna Landenberger; Michael Buhl; Efdal Yoeruek; Karl Ulrich Bartz-Schmidt; Matthias Bramkamp; Gunnar Blumenstock; Daniel Röck Journal: Medicine (Baltimore) Date: 2018-09 Impact factor: 1.817