Literature DB >> 2826402

Direct repeats flanking the Bacteroides transposon Tn4351 are insertion sequence elements.

V Hwa1, N B Shoemaker, A A Salyers.   

Abstract

The clindamycin-erythromycin resistance (Ccr Emr) region of the Bacteroides transposon Tn4351 is flanked by direct repeats. This study showed that the direct repeats are insertion sequence (IS) elements. Although both IS elements can mediate transfer of the chloramphenicol (Cmr) marker on pBR328 by cointegrate formation with the conjugal IncW plasmid R388, IS4351R-mediated transfer of Cmr occurred at a consistently lower frequency than did the transfer mediated by IS4351L. Analysis of plasmids from the resultant transconjugants revealed IS-mediated activities such as deletions, tandem duplication of IS4351L, and excision of IS4351R.

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Year:  1988        PMID： 2826402      PMCID： PMC210665          DOI： 10.1128/jb.170.1.449-451.1988

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  11 in total

1.  Mapping of functions in the R-plasmid R388 by examination of deletion mutants generated in vitro.

Authors:  J M Ward; J Grinsted
Journal:  Gene       Date:  1978-04       Impact factor: 3.688

2.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

3.  Complete nucleotide sequence of insertion element IS4351 from Bacteroides fragilis.

Authors:  J L Rasmussen; D A Odelson; F L Macrina
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

4.  Transposition of Tn4551 in Bacteroides fragilis: identification and properties of a new transposon from Bacteroides spp.

Authors:  C J Smith; H Spiegel
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

5.  Comparison of the transposon-like structures encoding clindamycin resistance in Bacteroides R-plasmids.

Authors:  C J Smith; M A Gonda
Journal:  Plasmid       Date:  1985-05       Impact factor: 3.466

6.  Characterization of Bacteroides ovatus plasmid pBI136 and structure of its clindamycin resistance region.

Authors:  C J Smith
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490

7.  Tn4351 transposes in Bacteroides spp. and mediates the integration of plasmid R751 into the Bacteroides chromosome.

Authors:  N B Shoemaker; C Getty; J F Gardner; A A Salyers
Journal:  J Bacteriol       Date:  1986-03       Impact factor: 3.490

8.  Expression in Escherichia coli of cryptic tetracycline resistance genes from bacteroides R plasmids.

Authors:  D G Guiney; P Hasegawa; C E Davis
Journal:  Plasmid       Date:  1984-05       Impact factor: 3.466

9.  Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.

Authors:  X Soberon; L Covarrubias; F Bolivar
Journal:  Gene       Date:  1980-05       Impact factor: 3.688

10.  Tn4400, a compound transposon isolated from Bacteroides fragilis, functions in Escherichia coli.

Authors:  N J Robillard; F P Tally; M H Malamy
Journal:  J Bacteriol       Date:  1985-12       Impact factor: 3.490
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  12 in total

1.  Cloning and partial characterization of two chromosomal loci from Bacteroides ovatus that contain genes essential for growth on guar gum.

Authors:  P J Valentine; P Arnold; A A Salyers
Journal:  Appl Environ Microbiol       Date:  1992-05       Impact factor: 4.792

2.  Identification of a second endogenous Porphyromonas gingivalis insertion element.

Authors:  C Y Wang; V C Bond; C A Genco
Journal:  J Bacteriol       Date:  1997-06       Impact factor: 3.490

3.  Mobilization of Bacteroides plasmids by Bacteroides conjugal elements.

Authors:  P J Valentine; N B Shoemaker; A A Salyers
Journal:  J Bacteriol       Date:  1988-03       Impact factor: 3.490

4.  Development of techniques to genetically manipulate members of the genera Cytophaga, Flavobacterium, Flexibacter, and Sporocytophaga.

Authors:  M J McBride; S A Baker
Journal:  Appl Environ Microbiol       Date:  1996-08       Impact factor: 4.792

5.  Importance of mucopolysaccharides as substrates for Bacteroides thetaiotaomicron growing in intestinal tracts of exgermfree mice.

Authors:  A A Salyers; M Pajeau; R E McCarthy
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792

6.  Analysis of proteins associated with growth of Bacteroides ovatus on the branched galactomannan guar gum.

Authors:  P J Valentine; A A Salyers
Journal:  Appl Environ Microbiol       Date:  1992-05       Impact factor: 4.792

7.  Development of techniques for the genetic manipulation of the gliding bacterium Cytophaga johnsonae.

Authors:  M J McBride; M J Kempf
Journal:  J Bacteriol       Date:  1996-02       Impact factor: 3.490

8.  Resistance of a Tn4351-generated polysaccharide mutant of Porphyromonas gingivalis to polymorphonuclear leukocyte killing.

Authors:  C A Genco; R E Schifferle; T Njoroge; R Y Forng; C W Cutler
Journal:  Infect Immun       Date:  1995-02       Impact factor: 3.441

9.  Cloning and characterization of a Bacteroides conjugal tetracycline-erythromycin resistance element by using a shuttle cosmid vector.

Authors:  N B Shoemaker; R D Barber; A A Salyers
Journal:  J Bacteriol       Date:  1989-03       Impact factor: 3.490

10.  Characterization of a Tn4351-generated hemin uptake mutant of Porphyromonas gingivalis: evidence for the coordinate regulation of virulence factors by hemin.

Authors:  C A Genco; W Simpson; R Y Forng; M Egal; B M Odusanya
Journal:  Infect Immun       Date:  1995-07       Impact factor: 3.441
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