Single-Chain Polymeric Nanocarriers: A Platform for Determining Structure-Function Correlations in the Delivery of Molecular Cargo.

There has been growing interest in producing stable, biocompatible nanocarriers for the controlled delivery of therapeutics. With micelles, it remains a challenge to predict a priori the size, aggregation number, and functionality of the self-assembled aggregates. Utilizing controlled radical polymerization techniques, we have prepared tunable high molecular weight amphiphilic comb copolymers that self-assemble into unimolecular "micelle-like" nanocarriers of predictable size and functionality. Excellent control over self-assembly behavior and structure allows for systematic determination of the role of important polymeric material properties (i.e., glass transition) on the release of model therapeutics while simultaneously controlling for size, dispersity, structural, and functionality effects. Moreover, these single-chain polymeric nanocarriers represent a class of drug delivery systems allowing for interrogation of the limitations of standard methods for characterization of micellar aggregates.