AIM: Conventional disinfection techniques, considered safe for plasma, are usually associated with collateral damages on concentrated platelets and erythrocytes. Alternative methods are required and antimicrobial photodynamic therapy (aPDT) seems promising. In this study the effectiveness of two photosensitizers (PS), a porphyrin and a phthalocyanine, to disinfect blood products was evaluated. RESULTS: The cationic porphyrin was more effective in the photoinactivation of bacteria. Also, no significant osmotic stress was found for samples treated with PS at 5.0 µM in isotonic conditions after antimicrobial photodynamic therapy. CONCLUSION: Effective reduction of Gram-positive bacteria at 5.0 µM of PS provided promising indications toward its safe use to disinfect blood samples. For Gram-negative bacteria, lower PS concentrations, between 5.0 and 10 µM, must be tested.
AIM: Conventional disinfection techniques, considered safe for plasma, are usually associated with collateral damages on concentrated platelets and erythrocytes. Alternative methods are required and antimicrobial photodynamic therapy (aPDT) seems promising. In this study the effectiveness of two photosensitizers (PS), a porphyrin and a phthalocyanine, to disinfect blood products was evaluated. RESULTS: The cationic porphyrin was more effective in the photoinactivation of bacteria. Also, no significant osmotic stress was found for samples treated with PS at 5.0 µM in isotonic conditions after antimicrobial photodynamic therapy. CONCLUSION: Effective reduction of Gram-positive bacteria at 5.0 µM of PS provided promising indications toward its safe use to disinfect blood samples. For Gram-negative bacteria, lower PS concentrations, between 5.0 and 10 µM, must be tested.
Authors: Cátia Vieira; Ana T P C Gomes; Mariana Q Mesquita; Nuno M M Moura; M Graça P M S Neves; M Amparo F Faustino; Adelaide Almeida Journal: Front Microbiol Date: 2018-11-19 Impact factor: 5.640
Authors: Ana F R Cerqueira; Nuno M M Moura; Vanda Vaz Serra; M Amparo F Faustino; Augusto C Tomé; José A S Cavaleiro; M Graça P M S Neves Journal: Molecules Date: 2017-07-29 Impact factor: 4.411
Authors: Vera Sousa; Ana T P C Gomes; Américo Freitas; Maria A F Faustino; Maria G P M S Neves; Adelaide Almeida Journal: Antibiotics (Basel) Date: 2019-11-13
Authors: Kelly A D F Castro; Juliana A Prandini; Juliana Cristina Biazzotto; João P C Tomé; Roberto S da Silva; Leandro M O Lourenço Journal: Front Chem Date: 2022-03-14 Impact factor: 5.221
Authors: Patrícia Foletto; Fabiula Correa; Luciano Dornelles; Bernardo A Iglesias; Carolina H da Silveira; Pablo A Nogara; João B T da Rocha; Maria A F Faustino; Oscar E D Rodrigues Journal: Molecules Date: 2018-10-10 Impact factor: 4.411