Sima Tokajian1, Tamara Salloum1, Jonathan A Eisen2, Guillaume Jospin2, Anna Farra3, Jacques E Mokhbat3, David A Coil2. 1. School of Arts & Sciences, Department of Natural Sciences, Lebanese American University, Byblos, Lebanon. 2. Department of Evolution and Ecology, University of California Davis Genome Center, Davis, CA 95616, USA. 3. School of Medicine, Lebanese American University, Byblos, Lebanon.
Abstract
AIM: Extended-spectrum β-lactamase-producing (ESBL) Escherichia coli are a public threat worldwide. This study aimed at analyzing the genomic and functional attributes of nine ESBLs taken from rectal swabs. MATERIALS & METHODS: Samples were isolated from patients admitted for gastrointestinal and urological procedures at the University Medical Center-Rizk Hospital (UMCRH) in Lebanon. Illumina paired-end libraries were prepared and sequenced. RESULTS: The isolates were distributed into five lineages: ST131, ST648, ST405, ST73 and ST38, and harbored bla OXA-1, bla TEM-1B, bla TEM-1C and aac(6')Ib-cr. ST131 isolates were carriers of stx2 converting I phage. CONCLUSION: This is the first comprehensive genomic analysis performed on ESBLs in Lebanon.
AIM: Extended-spectrum β-lactamase-producing (ESBL) Escherichia coli are a public threat worldwide. This study aimed at analyzing the genomic and functional attributes of nine ESBLs taken from rectal swabs. MATERIALS & METHODS: Samples were isolated from patients admitted for gastrointestinal and urological procedures at the University Medical Center-Rizk Hospital (UMCRH) in Lebanon. Illumina paired-end libraries were prepared and sequenced. RESULTS: The isolates were distributed into five lineages: ST131, ST648, ST405, ST73 and ST38, and harbored bla OXA-1, bla TEM-1B, bla TEM-1C and aac(6')Ib-cr. ST131 isolates were carriers of stx2 converting I phage. CONCLUSION: This is the first comprehensive genomic analysis performed on ESBLs in Lebanon.
Authors: Supathep Tansirichaiya; Richard N Goodman; Xinyu Guo; Issra Bulgasim; Ørjan Samuelsen; Mohammed Al-Haroni; Adam P Roberts Journal: Microbiol Spectr Date: 2022-01-19