Thierry Olivry1,2, Stanley M Dunston1, Claude Favrot3, Pascal Prélaud4, Toshiroh Tsukui5. 1. Department of Clinical Sciences, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA. 2. Comparative Medicine Institute, NC State University, Raleigh, NC, 27606, USA. 3. Clinic for Small Animal Internal Medicine, Dermatology Department, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 260, CH-8057, Zürich, Switzerland. 4. Clinique Vétérinaire Advetia, 5 Rue Dubrunfaut, F-75012, Paris, France. 5. Animal Life Science Laboratories, Nippon Zenyaku Kogyo Corporation (Zenoaq), 1-1 Tairanoue, Sasagawa, Asaka-machi, Koriyama, Fukushima, 963-0196, Japan.
Abstract
BACKGROUND: Atopic dogs with hypersensitivity to Dermatophagoides farinae (Df) have IgE recognizing high molecular weight (MW) allergens more often than the low MW Der f 1 and 2. A new high MW Df allergen, Zen-1, has been identified recently. OBJECTIVES: To determine the IgE reactivity of American and European Df-hypersensitive dogs to Zen-1, Der f 1 and Der f 2. METHODS: We tested sera from 33 Df-reactive dogs from the USA, 29 from Europe and 15 experimentally sensitized to Df, by ELISA against crude Df, Der f 1, Der f 2 and Zen-1. ELISA inhibition was performed with sera reactive to Zen-1. Intradermal testing (IDT) was also done with the same allergens in 25 other American atopic dogs. RESULTS: Altogether, IgE seropositivity to Zen-1 was more prevalent (86%) than that to Der f 1 (17%) or Der f 2 (19%). The IgE reactivity to Zen-1 was correlated to that against crude Df; this allergen alone inhibited a high percentage (median: 50%; range: 22-84%) of the binding to the crude mite extract. The seropositivity to low MW allergens was highest in experimentally sensitized dogs. Serum IgE recognition of Der f 1 was low in dogs with AD; that to Der f 2 was significantly lower in American dogs (6%) than in European ones (28%). A high prevalence of positive immediate IDT reactions to Zen-1 confirmed the likely relevance of serological results. CONCLUSIONS AND CLINICAL IMPORTANCE: This study establishes Zen-1 as a major allergen in atopic dogs sensitized to Df.
BACKGROUND: Atopic dogs with hypersensitivity to Dermatophagoides farinae (Df) have IgE recognizing high molecular weight (MW) allergens more often than the low MW Der f 1 and 2. A new high MW Df allergen, Zen-1, has been identified recently. OBJECTIVES: To determine the IgE reactivity of American and European Df-hypersensitivedogs to Zen-1, Der f 1 and Der f 2. METHODS: We tested sera from 33 Df-reactive dogs from the USA, 29 from Europe and 15 experimentally sensitized to Df, by ELISA against crude Df, Der f 1, Der f 2 and Zen-1. ELISA inhibition was performed with sera reactive to Zen-1. Intradermal testing (IDT) was also done with the same allergens in 25 other American atopic dogs. RESULTS: Altogether, IgE seropositivity to Zen-1 was more prevalent (86%) than that to Der f 1 (17%) or Der f 2 (19%). The IgE reactivity to Zen-1 was correlated to that against crude Df; this allergen alone inhibited a high percentage (median: 50%; range: 22-84%) of the binding to the crude mite extract. The seropositivity to low MW allergens was highest in experimentally sensitized dogs. Serum IgE recognition of Der f 1 was low in dogs with AD; that to Der f 2 was significantly lower in American dogs (6%) than in European ones (28%). A high prevalence of positive immediate IDT reactions to Zen-1 confirmed the likely relevance of serological results. CONCLUSIONS AND CLINICAL IMPORTANCE: This study establishes Zen-1 as a major allergen in atopic dogs sensitized to Df.
Authors: Stephanie B Abrams; Guy N Brock; Marilly Palettas; Michelle L Bolner; Tricia Moore-Sowers; Greg A Plunkett; Lynette K Cole; Sandra F Diaz; Gwendolen Lorch Journal: Vet Dermatol Date: 2018-09-03 Impact factor: 1.867
Authors: Raffaela Campana; Sheron Dzoro; Irene Mittermann; Elena Fedenko; Olga Elisyutina; Musa Khaitov; Alexander Karaulov; Rudolf Valenta Journal: Curr Opin Allergy Clin Immunol Date: 2017-08