Literature DB >> 28260495

Immuno-PCR: Achievements and Perspectives.

D Y Ryazantsev1, D V Voronina, S K Zavriev.   

Abstract

The immuno-PCR (iPCR) method combines advantages of enzyme-linked immunosorbent assay and polymerase chain reaction, which is used in iPCR as a method of "visualization" of antigen-antibody interaction. The use of iPCR provides classical PCR sensitivity to objects traditionally detected by ELISA. This method could be very sensitive and allow for detection of quantities of femtograms/ml order. However, iPCR is still not widely used. The aim of this review is to highlight the special features of the iPCR method and to show the main aspects of its development and application in recent years.

Entities:  

Mesh：

Substances：

Year:  2016        PMID： 28260495     DOI： 10.1134/S0006297916130113

Source DB:  PubMed          Journal:  Biochemistry (Mosc)        ISSN： 0006-2979            Impact factor:   2.487


  7 in total

1.  Tandem blocking of PCR extension to form a single-stranded overhang for facile, visual, and ultrasensitive gene detection.

Authors:  Zhe Sui; Tong Li; Ran An; Wei Wu; Makoto Komiyama; Xingguo Liang
Journal:  RSC Adv       Date:  2018-04-25       Impact factor: 4.036

Review 2.  Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research.

Authors:  Helena Kupcova Skalnikova; Jana Cizkova; Jakub Cervenka; Petr Vodicka
Journal:  Int J Mol Sci       Date:  2017-12-13       Impact factor: 5.923

3.  Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells.

Authors:  Jessie A G van Buggenum; Jan P Gerlach; Sabine E J Tanis; Mark Hogeweg; Pascal W T C Jansen; Jesse Middelwijk; Ruud van der Steen; Michiel Vermeulen; Hendrik G Stunnenberg; Cornelis A Albers; Klaas W Mulder
Journal:  Nat Commun       Date:  2018-06-19       Impact factor: 14.919

4.  Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR.

Authors:  Yi Su; Wei Li; Zhigang Huang; Ruozhong Wang; Weigui Luo; Qing Liu; Jianhua Tong; Langtao Xiao
Journal:  Plant Methods       Date:  2018-11-24       Impact factor: 4.993

Review 5.  Uncovering the Depths of the Human Proteome: Antibody-based Technologies for Ultrasensitive Multiplexed Protein Detection and Quantification.

Authors:  Annie H Ren; Eleftherios P Diamandis; Vathany Kulasingam
Journal:  Mol Cell Proteomics       Date:  2021-09-28       Impact factor: 7.381

6.  Exploring solid-phase proximity ligation assay for survivin detection in urine.

Authors:  Jan Gleichenhagen; Christian Arndt; Swaantje Casjens; Carmen Töpfer; Holger Gerullis; Irina Raiko; Dirk Taeger; Thorsten Ecke; Thomas Brüning; Georg Johnen
Journal:  PLoS One       Date:  2022-06-29       Impact factor: 3.752

7.  Laser Capture Microdissection Coupled Capillary Immunoassay to Study the Expression of PCK-2 on Spatially-Resolved Islets of Rat Langerhans.

Authors:  Shashank Pandey; Zdenek Tuma; Tereza Smrhova; Miroslava Cedikova; Tereza Macanova; Magdalena Chottova Dvorakova
Journal:  Pharmaceutics       Date:  2021-06-15       Impact factor: 6.321
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.