Literature DB >> 28260146

Simultaneous detection and identification of STI pathogens by multiplex Real-Time PCR in genital tract specimens in a selected area of Apulia, a region of Southern Italy.

Raffaele Del Prete1,2, Luigi Ronga3, Mirella Lestingi4, Grazia Addati4, Umberto Filippo Angelotti4, Domenico Di Carlo5, Giuseppe Miragliotta4,3.   

Abstract

PURPOSE: Genital tract infections are globally a major cause of morbidity in sexually active individuals. The aim of this study was to investigate the prevalence and associations of co-infections of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma hominis (MH), Mycoplasma genitalium, Ureaplasma urealyticum (UU) and Ureaplasma parvum (UP) in specimens collected from female (SF) and male (SM) patients.
METHODS: 1575 samples from 1575 individuals from the geographical area around Bari, Apulia region in Southern Italy, were collected and analyzed by a multiplex Real-Time PCR (mRT-PCR) (AnyplexTM II STI-7, Seegene, Inc., Seoul, Korea) assay.
RESULTS: 455/1575 (28.89%) samples resulted positive for at least one of the targets named above. Statistically significant differences in prevalence of the pathogens between SF and SM were not detected except for UP (24.92% in SF vs 8.91% in SM). Prevalence of co-infections was 6.84 and 3.96% in SF and SM, respectively. Moreover, MH presence in SF, but not in SM, was associated with UU and UP.
CONCLUSIONS: Our data suggest different patterns of infections between females and male and the importance of an increased vigilance of sexually transmitted pathogens to reduce the burden on general population and the sequelae or the complications on reproductive organs.

Entities:  

Keywords:  Co-infections; Genital tract; Multiplex Real-Time PCR; Sexually transmitted infections

Mesh:

Year:  2017        PMID: 28260146     DOI: 10.1007/s15010-017-1002-7

Source DB:  PubMed          Journal:  Infection        ISSN: 0300-8126            Impact factor:   3.553


  34 in total

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Authors:  Marita J W van de Laar; Servaas A Morré
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Authors:  M M Rodrigues; P Á Fernandes; J P Haddad; M C Paiva; M Do Carmo M Souza; T C A Andrade; A P Fernandes
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Journal:  J Matern Fetal Neonatal Med       Date:  2013-07-18

Review 5.  Mycoplasmas and ureaplasmas as neonatal pathogens.

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Journal:  Clin Microbiol Rev       Date:  2005-10       Impact factor: 26.132

6.  Clinical relevance of Ureaplasma urealyticum colonization in preterm infants.

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8.  Detection of Ureaplasma biovars and polymerase chain reaction-based subtyping of Ureaplasma parvum in women with or without symptoms of genital infections.

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