| Literature DB >> 28258211 |
Tianyuan Chen1,2,3, Dan Liu1,2,3, Xiaolin Niu1,2,3, Junzhu Wang1,2,3, Lichao Qian1,2,3, Lu Han1,2,3, Na Liu1,2,3, Jinping Zhao1,2,3, Yiguo Hong1,2,3, Yule Liu4,5,6.
Abstract
The tomato Tobacco mosaic virus resistance-22 (Tm-22 ) gene encodes a coiled-coil-nucleotide binding site-Leu-rich repeat protein lacking a conventional plasma membrane (PM) localization motif. Tm-22 confers plant extreme resistance against tobamoviruses including Tobacco mosaic virus (TMV) by recognizing the avirulence (Avr) viral movement protein (MP). However, the subcellular compartment where Tm-22 functions is unclear. Here, we demonstrate that Tm-22 interacts with TMV MP to form a protein complex at the PM We show that both inactive and active Tm-22 proteins are localized to the PM When restricted to PM by fusing Tm-22 to the S-acylated PM association motif, the Tm-22 fusion protein can still induce a hypersensitive response cell death, consistent with its activation at the PM Through analyses of viral MP mutants, we find that the plasmodesmata (PD) localization of the Avr protein MP is not required for Tm-22 function. These results suggest that Tm-22-mediated resistance takes place on PM without requirement of its Avr protein to be located to PD.Entities:
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Year: 2017 PMID： 28258211 PMCID： PMC5373048 DOI： 10.1104/pp.16.01512
Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340