Varun Sharma1, Indu Sharma1, Itty Sethi1, Ankit Mahajan2, Gurvinder Singh3, Arshia Angural1, A J S Bhanwer3, Manoj K Dhar2, Vinod Singh1, Ekta Rai4, Swarkar Sharma5. 1. Human Genetics Research Group, Department of Biotechnology, Shri Mata Vaishno Devi University, Katra, 182320, India. 2. Department of Biotechnology, University of Jammu, 180006, India. 3. Department of Human Genetics, Guru Nanak Dev University, Amritsar, India. 4. Human Genetics Research Group, Department of Biotechnology, Shri Mata Vaishno Devi University, Katra, 182320, India. Electronic address: ekta.rai@smvdu.ac.in. 5. Human Genetics Research Group, Department of Biotechnology, Shri Mata Vaishno Devi University, Katra, 182320, India. Electronic address: Swarkar.sharma@smvdu.ac.in.
Abstract
OBJECTIVE: To replicate the association of newly identified variants of TMEM163 (transmembrane protein 163) and COBLL1 (cordon-bleu protein-like 1) with type 2 diabetes (T2D) in Northwest Indian population. METHODS: We performed a replication study of variants rs998451 and rs6723108 of gene TMEM163 and rs7607980 of gene COBLL1. The variations were genotyped using Taqman allele discrimination assay in 1209 Northwest Indians (651 T2D cases and 558 controls). The association of each SNP with the disease was evaluated using logistic regression. RESULTS: All the three SNPs examined in this study did not show any significant association with T2D. For rs998451 and rs6723108 of TMEM163 the observed odds ratios were 0.71 with a 95% CI of 0.28-1.84 (p=0.484) and 1.80 with a 95% CI of 0.74-4.40 (p=0.196), respectively. For rs7607980 the estimated odds ratio was 1.01 with 95% CI of 0.70-1.44 (p=0.946). CONCLUSION: We conclude that lack of association could be because of population structure of Indian Population that is conglomeration of various ethnic groups. For a conclusive association study of T2D in India, it is critical that such studies are carried out among endogamous ethnic groups rather than conventional practice of pooling samples based on Geographical/regional or linguist affiliations like Asian Indian, North or South Indian etc.
OBJECTIVE: To replicate the association of newly identified variants of TMEM163 (transmembrane protein 163) and COBLL1 (cordon-bleu protein-like 1) with type 2 diabetes (T2D) in Northwest Indian population. METHODS: We performed a replication study of variants rs998451 and rs6723108 of gene TMEM163 and rs7607980 of gene COBLL1. The variations were genotyped using Taqman allele discrimination assay in 1209 Northwest Indians (651 T2D cases and 558 controls). The association of each SNP with the disease was evaluated using logistic regression. RESULTS: All the three SNPs examined in this study did not show any significant association with T2D. For rs998451 and rs6723108 of TMEM163 the observed odds ratios were 0.71 with a 95% CI of 0.28-1.84 (p=0.484) and 1.80 with a 95% CI of 0.74-4.40 (p=0.196), respectively. For rs7607980 the estimated odds ratio was 1.01 with 95% CI of 0.70-1.44 (p=0.946). CONCLUSION: We conclude that lack of association could be because of population structure of Indian Population that is conglomeration of various ethnic groups. For a conclusive association study of T2D in India, it is critical that such studies are carried out among endogamous ethnic groups rather than conventional practice of pooling samples based on Geographical/regional or linguist affiliations like Asian Indian, North or South Indian etc.
Authors: Przemysław Ustianowski; Damian Malinowski; Krzysztof Safranow; Violetta Dziedziejko; Maciej Tarnowski; Andrzej Pawlik Journal: J Pers Med Date: 2022-02-08
Authors: Przemyslaw Ustianowski; Damian Malinowski; Michał Czerewaty; Krzysztof Safranow; Maciej Tarnowski; Violetta Dziedziejko; Andrzej Pawlik Journal: Biomedicines Date: 2022-08-09