Literature DB >> 28257471

Correction: Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA Genes.

Tatsuhiko Hoshino, Fumio Inagaki.   

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0169431.].

Year:  2017        PMID: 28257471      PMCID: PMC5336281          DOI: 10.1371/journal.pone.0173546

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


There are errors in the fourth and fifth sentences of the Quantification by qSeq subsection of the Materials and Methods section. The correct sentences are: The SPE conditions consisted of initial denaturation at 98°C for 2 min, cooling to 55°C at 0.3°C/s, and elongation at 68°C for 10 min. Subsequently, excess primers were digested by addition of 4 μl of Exonuclease I (5 U/μl, TaKaRa Bio) and incubation at 37°C for 120 min, followed by inactivation of the enzyme by incubation at 80°C for 30 min.
  1 in total

1.  Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA Genes.

Authors:  Tatsuhiko Hoshino; Fumio Inagaki
Journal:  PLoS One       Date:  2017-01-04       Impact factor: 3.240

  1 in total
  1 in total

1.  Abundance and Diversity of Hydrogenotrophic Microorganisms in the Infant Gut before the Weaning Period Assessed by Denaturing Gradient Gel Electrophoresis and Quantitative PCR.

Authors:  Valeria Sagheddu; Vania Patrone; Francesco Miragoli; Lorenzo Morelli
Journal:  Front Nutr       Date:  2017-06-26
  1 in total

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