Literature DB >> 28255573

Evaluation of Burkholderia cepacia Complex Bacteria Pathogenicity Using Caenorhabditis elegans.

Pietro Tedesco1, Elia Di Schiavi2, Fortunato Palma Esposito1, Donatella de Pascale1.   

Abstract

This protocol describes two biological assays to evaluate pathogenicity of Burkholderia cepacia complex (Bcc) strains against the nematode Caenorhabditis elegans. Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing assay, SKA) or by toxins production (fast killing assay, FKA). The principal differences between the two assays rely on the different killing kinetics for worms.

Entities:  

Year:  2016        PMID： 28255573      PMCID： PMC5330310          DOI： 10.21769/BioProtoc.1964

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  4 in total

Review 1.  Maintenance of C. elegans.

Authors:  Theresa Stiernagle
Journal:  WormBook       Date:  2006-02-11

2.  Diverse pathogenicity of Burkholderia cepacia complex strains in the Caenorhabditis elegans host model.

Authors:  Silvia T Cardona; Julia Wopperer; Leo Eberl; Miguel A Valvano
Journal:  FEMS Microbiol Lett       Date:  2005-09-01       Impact factor: 2.742

3.  Killing of Caenorhabditis elegans by Burkholderia cepacia is controlled by the cep quorum-sensing system.

Authors:  Manuela Köthe; Melanie Antl; Birgit Huber; Kilian Stoecker; Doreen Ebrecht; Ivo Steinmetz; Leo Eberl
Journal:  Cell Microbiol       Date:  2003-05       Impact factor: 3.715

4.  Investigating the Role of the Host Multidrug Resistance Associated Protein Transporter Family in Burkholderia cepacia Complex Pathogenicity Using a Caenorhabditis elegans Infection Model.

Authors:  Pietro Tedesco; Marco Visone; Ermenegilda Parrilli; Maria Luisa Tutino; Elena Perrin; Isabel Maida; Renato Fani; Francesco Ballestriero; Radleigh Santos; Clemencia Pinilla; Elia Di Schiavi; George Tegos; Donatella de Pascale
Journal:  PLoS One       Date:  2015-11-20       Impact factor: 3.240
  4 in total
  2 in total

1.  Mutation of the cyclic di-GMP phosphodiesterase gene in Burkholderia lata SK875 attenuates virulence and enhances biofilm formation.

Authors:  Hae-In Jung; Yun-Jung Kim; Yun-Jung Lee; Hee-Soo Lee; Jung-Kee Lee; Soo-Ki Kim
Journal:  J Microbiol       Date:  2017-09-28       Impact factor: 3.422

2.  Characterization of Nematicidal Activity and Nematode-Toxic Metabolites of a Soilborne Brevundimonas bullata Isolate.

Authors:  Jiaoqing Li; Meijuan Ding; Xiaowen Sun; Zhe Li; Liangzheng Xu; Lin Li
Journal:  Pathogens       Date:  2022-06-20
  2 in total

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