Literature DB >> 2825487

Fluorometer based multi-parameter analysis of phagocytic cell activation.

B Seligmann1, K Patel, W O Haston, J J Rediske.   

Abstract

Simultaneous measurements of the calcium rise, membrane potential change, and 90 degrees light scatter (shape change) responses exhibited by neutrophils upon activation, can be obtained with identical result as that obtained when independently performing each measurement. The putative intracellular mediator diacylglycerol depolarizes membrane potential and causes a decrease in light scatter. Leukotriene B4 causes a rise in calcium and a decrease in light scatter. The chemotactic peptide, N-formylmethionyl-leucyl-phenylalanine, causes a depolarization of membrane potential, a calcium rise, and a decrease in light scatter. The fura 2 measurements of intracellular free calcium indicate that the calcium concentration of unstimulated cells is much lower than previously thought based on quin 2 studies.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 2825487     DOI： 10.1007/BF01966520

Source DB:  PubMed          Journal:  Agents Actions        ISSN： 0065-4299


  6 in total

1.  A new generation of Ca2+ indicators with greatly improved fluorescence properties.

Authors:  G Grynkiewicz; M Poenie; R Y Tsien
Journal:  J Biol Chem       Date:  1985-03-25       Impact factor: 5.157

2.  Use of lipophilic probes of membrane potential to assess human neutrophil activation. Abnormality in chronic granulomatous disease.

Authors:  B E Seligmann; J I Gallin
Journal:  J Clin Invest       Date:  1980-09       Impact factor: 14.808

3.  Correlation between membrane potential changes and superoxide production in human granulocytes stimulated by phorbol myristate acetate. Evidence for defective activation in chronic granulomatous disease.

Authors:  J C Whitin; C E Chapman; E R Simons; M E Chovaniec; H J Cohen
Journal:  J Biol Chem       Date:  1980-03-10       Impact factor: 5.157

4.  Comparison of indirect probes of membrane potential utilized in studies of human neutrophils.

Authors:  B E Seligmann; J I Gallin
Journal:  J Cell Physiol       Date:  1983-05       Impact factor: 6.384

5.  Relationship of actin polymerization and depolymerization to light scattering in human neutrophils: dependence on receptor occupancy and intracellular Ca++.

Authors:  L A Sklar; G M Omann; R G Painter
Journal:  J Cell Biol       Date:  1985-09       Impact factor: 10.539

6.  Modulation of cytosolic-free calcium transients by changes in intracellular calcium-buffering capacity: correlation with exocytosis and O2-production in human neutrophils.

Authors:  P D Lew; C B Wollheim; F A Waldvogel; T Pozzan
Journal:  J Cell Biol       Date:  1984-10       Impact factor: 10.539
  6 in total
  2 in total

1.  Solution structure of a unique C5a semi-synthetic antagonist: implications in receptor binding.

Authors:  X Zhang; W Boyar; N Galakatos; N C Gonnella
Journal:  Protein Sci       Date:  1997-01       Impact factor: 6.725

2.  The pharmacophore of the human C5a anaphylatoxin.

Authors:  M J Toth; L Huwyler; W C Boyar; A F Braunwalder; D Yarwood; J Hadala; W O Haston; M A Sills; B Seligmann; N Galakatos
Journal:  Protein Sci       Date:  1994-08       Impact factor: 6.725
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.