Literature DB >> 28252199

Analysis of mtDNA/nDNA Ratio in Mice.

Pedro M Quiros1, Aashima Goyal1, Pooja Jha1, Johan Auwerx1.   

Abstract

Mitochondrial DNA (mtDNA) lacks the protection provided by the nucleosomes in the nuclear DNA and does not have a DNA repair mechanism, making it highly susceptible to damage, which can lead to mtDNA depletion. mtDNA depletion compromises the efficient function of cells and tissues and thus impacts negatively on health. Here, we describe a brief and easy protocol to quantify mtDNA copy number by determining the mtDNA/nDNA ratio. The procedure has been validated using a cohort of young and aged mice. © 2017 by John Wiley & Sons, Inc.
Copyright © 2017 John Wiley & Sons, Inc.

Entities:  

Keywords:  16SrRNA; ND1; mtDNA; qPCR

Mesh：

Substances：

Year:  2017        PMID： 28252199      PMCID： PMC5335900          DOI： 10.1002/cpmo.21

Source DB:  PubMed          Journal:  Curr Protoc Mouse Biol        ISSN： 2161-2617


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