Literature DB >> 28248351

Extended depth-of-field microscopy with a high-speed deformable mirror.

William J Shain, Nicholas A Vickers, Bennett B Goldberg, Thomas Bifano, Jerome Mertz.   

Abstract

We present a wide-field fluorescence microscopy add-on that provides a fast, light-efficient extended depth-of-field (EDOF) using a deformable mirror with an update rate of 20 kHz. Out-of-focus contributions in the raw EDOF images are suppressed with a deconvolution algorithm derived directly from the microscope 3D optical transfer function. Demonstrations of the benefits of EDOF microscopy are shown with GCaMP-labeled mouse brain tissue.

Entities:  

Year:  2017        PMID： 28248351     DOI： 10.1364/OL.42.000995

Source DB:  PubMed          Journal:  Opt Lett        ISSN： 0146-9592            Impact factor:   3.776


  25 in total

1.  Five-dimensional two-photon volumetric microscopy of in-vivo dynamic activities using liquid lens remote focusing.

Authors:  Kayvan Forouhesh Tehrani; Charles V Latchoumane; W Michael Southern; Emily G Pendleton; Ana Maslesa; Lohitash Karumbaiah; Jarrod A Call; Luke J Mortensen
Journal:  Biomed Opt Express       Date:  2019-06-26       Impact factor: 3.732

2.  Fast, multiplane line-scan confocal microscopy using axially distributed slits.

Authors:  Jean-Marc Tsang; Howard J Gritton; Shoshana L Das; Timothy D Weber; Christopher S Chen; Xue Han; Jerome Mertz
Journal:  Biomed Opt Express       Date:  2021-02-09       Impact factor: 3.732

3.  Reduction of spherical and chromatic aberration in axial-scanning optical systems with tunable lenses.

Authors:  James A Strother
Journal:  Biomed Opt Express       Date:  2021-05-19       Impact factor: 3.732

4.  Volumetric chemical imaging in vivo by a remote-focusing stimulated Raman scattering microscope.

Authors:  Peng Lin; Hongli Ni; Huate Li; Nicholas A Vickers; Yuying Tan; Ruyi Gong; Thomas Bifano; Ji-Xin Cheng
Journal:  Opt Express       Date:  2020-09-28       Impact factor: 3.894

5.  A MEMS lens scanner based on serpentine electrothermal bimorph actuators for large axial tuning.

Authors:  Liang Zhou; Xiaomin Yu; Philip X-L Feng; Jianhua Li; Huikai Xie
Journal:  Opt Express       Date:  2020-08-03       Impact factor: 3.894

6.  Axial localization with modulated-illumination extended-depth-of-field microscopy.

Authors:  William J Shain; Nicholas A Vickers; Jiang Li; Xue Han; Thomas Bifano; Jerome Mertz
Journal:  Biomed Opt Express       Date:  2018-03-21       Impact factor: 3.732

7.  Optical volumetric projection for fast 3D imaging through circularly symmetric pupil engineering.

Authors:  Bo Cai; Xiaomin Zhai; Zeguan Wang; Yan Shen; Ronald Xu; Zachary J Smith; Quan Wen; Kaiqin Chu
Journal:  Biomed Opt Express       Date:  2018-01-04       Impact factor: 3.732

8.  Continuous focal translation enhances rate of point-scan volumetric microscopy.

Authors:  Courtney Johnson; Jack Exell; Jonathon Kuo; Kevin Welsher
Journal:  Opt Express       Date:  2019-12-09       Impact factor: 3.894

9.  Aberration-free multi-plane imaging of neural activity from the mammalian brain using a fast-switching liquid crystal spatial light modulator.

Authors:  Rui Liu; Neil Ball; James Brockill; Leonard Kuan; Daniel Millman; Cassandra White; Arielle Leon; Derric Williams; Shig Nishiwaki; Saskia de Vries; Josh Larkin; David Sullivan; Cliff Slaughterbeck; Colin Farrell; Peter Saggau
Journal:  Biomed Opt Express       Date:  2019-09-12       Impact factor: 3.732

10.  Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy.

Authors:  Siegfried Weisenburger; Frank Tejera; Jeffrey Demas; Brandon Chen; Jason Manley; Fraser T Sparks; Francisca Martínez Traub; Tanya Daigle; Hongkui Zeng; Attila Losonczy; Alipasha Vaziri
Journal:  Cell       Date:  2019-04-11       Impact factor: 41.582
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