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Literature DB >> 2824601

Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method.

C Y Li¹, S C Ziesmer, O Lazcano-Villareal.

Abstract

We describe a simple and effective method for inhibition of endogenous peroxidase activity in the immunoperoxidase technique. Specimens are pre-treated with a mixture of azide and hydrogen peroxide, which is then followed by an indirect immunoperoxidase procedure. Comparison studies showed no significant loss of antigenicity or morphological details by this pre-treatment. The method is most useful for evaluating cell-specific antigens on specimens that have abundant endogenous peroxidase activity, such as blood and bone marrow.

Entities: Chemical

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Year: 1987 PMID： 2824601 DOI： 10.1177/35.12.2824601

Source DB: PubMed Journal: J Histochem Cytochem ISSN： 0022-1554 Impact factor: 2.479

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Cited

37 in total

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2. Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders.

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3. Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging.

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Journal: J Histochem Cytochem Date: 2007-12-23 Impact factor: 2.479

4. Biochemical and immuno- and lectin-histochemical studies of solubility and retention of bone matrix proteins during EDTA demineralization.

Authors: M Takagi; M Maeno; Y Takahashi; K Otsuka
Journal: Histochem J Date: 1992-02

Review 5. Practical suggestions for successful immunoenzyme double-staining experiments.

Authors: C M van der Loos; A E Becker; J J van den Oord
Journal: Histochem J Date: 1993-01

Review 6. Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.

Authors: E J Speel; F C Ramaekers; A H Hopman
Journal: Histochem J Date: 1995-11

Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method.

1. Expression of guanylin in "pars tuberalis-specific cells" and gonadotrophs of rat adenohypophysis.

2. Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders.

3. Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging.

4. Biochemical and immuno- and lectin-histochemical studies of solubility and retention of bone matrix proteins during EDTA demineralization.

Review 5. Practical suggestions for successful immunoenzyme double-staining experiments.

Review 6. Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.

7. Disaccharides Protect Antigens from Drying-Induced Damage in Routinely Processed Tissue Sections.

8. Nature and distribution of chondroitin sulphate and dermatan sulphate proteoglycans in rabbit alveolar bone.

9. Localization of GAD-like immunoreactivity in the pancreas and stomach of the rat and mouse.

10. Immunohistochemical localization of the rabbit haemorrhagic disease viral antigen.