BACKGROUND: Inflammation is closely related to atrial fibrillation (AF) pathogenesis, and interleukin-37 (IL-37) represents a new member of the anti-inflammatory cytokines. HYPOTHESIS: IL-37 might play an important role in AF development and act as a potential risk factor for AF diagnosis. METHODS: The mRNA level of IL-37 in peripheral blood mononuclear cells (PBMCs) and serum IL-37 levels in AF patients and healthy controls were measured by real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). PBMCs from AF patients were stimulated with recombinant IL-37. Levels of pro-inflammatory cytokines IL-6 and C-reactive protein were determined by RT-PCR and ELISA. RESULTS: IL-37 mRNAs and serum protein levels were higher in patients with AF or lone AF compared with healthy controls. Patients with paroxysmal AF or persistent AF showed higher IL-37 mRNAs and serum protein levels compared with those with permanent AF as well as healthy controls. In vitro, IL-37 inhibited the production of IL-6 and C-reactive protein in PBMCs of patients with AF. CONCLUSIONS: IL-37 is elevated in AF patients and its expression is closely associated with AF subgroups. Thus, IL-37 may provide a novel research target for the pathogenesis and therapy of AF. This study is the first to document elevated IL-37 in AF patients.
BACKGROUND: Inflammation is closely related to atrial fibrillation (AF) pathogenesis, and interleukin-37 (IL-37) represents a new member of the anti-inflammatory cytokines. HYPOTHESIS: IL-37 might play an important role in AF development and act as a potential risk factor for AF diagnosis. METHODS: The mRNA level of IL-37 in peripheral blood mononuclear cells (PBMCs) and serum IL-37 levels in AFpatients and healthy controls were measured by real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). PBMCs from AFpatients were stimulated with recombinant IL-37. Levels of pro-inflammatory cytokines IL-6 and C-reactive protein were determined by RT-PCR and ELISA. RESULTS:IL-37 mRNAs and serum protein levels were higher in patients with AF or lone AF compared with healthy controls. Patients with paroxysmal AF or persistent AF showed higher IL-37 mRNAs and serum protein levels compared with those with permanent AF as well as healthy controls. In vitro, IL-37 inhibited the production of IL-6 and C-reactive protein in PBMCs of patients with AF. CONCLUSIONS:IL-37 is elevated in AFpatients and its expression is closely associated with AF subgroups. Thus, IL-37 may provide a novel research target for the pathogenesis and therapy of AF. This study is the first to document elevated IL-37 in AFpatients.
Authors: V Fuster; L E Rydén; R W Asinger; D S Cannom; H J Crijns; R L Frye; J L Halperin; G N Kay; W W Klein; S Lévy; R L McNamara; E N Prystowsky; L S Wann; D G Wyse; R J Gibbons; E M Antman; J S Alpert; D P Faxon; V Fuster; G Gregoratos; L F Hiratzka; A K Jacobs; R O Russell; S C Smith; W W Klein; A Alonso-Garcia; C Blomström-Lundqvist; G de Backer; M Flather; J Hradec; A Oto; A Parkhomenko; S Silber; A Torbicki Journal: Circulation Date: 2001-10-23 Impact factor: 29.690
Authors: M K Chung; D O Martin; D Sprecher; O Wazni; A Kanderian; C A Carnes; J A Bauer; P J Tchou; M J Niebauer; A Natale; D R Van Wagoner Journal: Circulation Date: 2001-12-11 Impact factor: 29.690
Authors: Thomas J Wang; Martin G Larson; Daniel Levy; Ramachandran S Vasan; Eric P Leip; Philip A Wolf; Ralph B D'Agostino; Joanne M Murabito; William B Kannel; Emelia J Benjamin Journal: Circulation Date: 2003-05-27 Impact factor: 29.690
Authors: Thomas J Wang; Joseph M Massaro; Daniel Levy; Ramachandran S Vasan; Philip A Wolf; Ralph B D'Agostino; Martin G Larson; William B Kannel; Emelia J Benjamin Journal: JAMA Date: 2003-08-27 Impact factor: 56.272
Authors: Ronnier J Aviles; David O Martin; Carolyn Apperson-Hansen; Penny L Houghtaling; Pentti Rautaharju; Richard A Kronmal; Russell P Tracy; David R Van Wagoner; Bruce M Psaty; Michael S Lauer; Mina K Chung Journal: Circulation Date: 2003-11-17 Impact factor: 29.690