Literature DB >> 28244041

Differentiation and Metabolic Interrogation of Human Adipocytes.

Nicki A Baker1, Lindsey A Muir2, Carey N Lumeng2, Robert W O'Rourke3,4.   

Abstract

Adipocytes differentiated from preadipocytes provide a valuable model for the study of human adipocyte metabolism. We describe methods for isolation of human stromal vascular cells, expansion of preadipocytes, differentiation into mature adipocytes, and in vitro metabolic interrogation of adipocytes.

Entities:  

Keywords:  Adipocyte; Adipose tissue; Differentiation; Glucose uptake; Lipogenesis; Lipolysis; Preadipocyte; Stromal vascular cell fraction

Mesh：

Substances：

Year:  2017        PMID： 28244041      PMCID： PMC5762179          DOI： 10.1007/978-1-4939-6820-6_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  26 in total

1.  Preadipocytes in the human subcutaneous adipose tissue display distinct features from the adult mesenchymal and hematopoietic stem cells.

Authors:  Coralie Sengenès; Karine Lolmède; Alexia Zakaroff-Girard; Rudi Busse; Anne Bouloumié
Journal:  J Cell Physiol       Date:  2005-10       Impact factor: 6.384

2.  A novel technique to propagate primary human preadipocytes without loss of differentiation capacity.

Authors:  Thomas Skurk; Silke Ecklebe; Hans Hauner
Journal:  Obesity (Silver Spring)       Date:  2007-12       Impact factor: 5.002

3.  Beneficial effects of subcutaneous fat transplantation on metabolism.

Authors:  Thien T Tran; Yuji Yamamoto; Stephane Gesta; C Ronald Kahn
Journal:  Cell Metab       Date:  2008-05       Impact factor: 27.287

4.  Characterization of the transcriptional and functional effects of fibroblast growth factor-1 on human preadipocyte differentiation.

Authors:  Felicity S Newell; Hua Su; Hans Tornqvist; Jonathan P Whitehead; Johannes B Prins; Louise J Hutley
Journal:  FASEB J       Date:  2006-10-26       Impact factor: 5.191

5.  Sex- and depot-dependent differences in adipogenesis in normal-weight humans.

Authors:  Yourka D Tchoukalova; Christina Koutsari; Susanne B Votruba; Tamara Tchkonia; Nino Giorgadze; Thomas Thomou; James L Kirkland; Michael D Jensen
Journal:  Obesity (Silver Spring)       Date:  2010-03-18       Impact factor: 5.002

6.  Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes.

Authors:  Thomas E Akie; Marcus P Cooper
Journal:  J Vis Exp       Date:  2015-08-27       Impact factor: 1.355

7.  Optimal protocol for the differentiation and metabolic analysis of human adipose stromal cells.

Authors:  Mi-Jeong Lee; Susan K Fried
Journal:  Methods Enzymol       Date:  2014       Impact factor: 1.600

8.  Effect of testosterone on lipolysis in human pre-adipocytes from different fat depots.

Authors:  A Dicker; M Rydén; E Näslund; I E Muehlen; M Wirén; M Lafontan; P Arner
Journal:  Diabetologia       Date:  2004-01-30       Impact factor: 10.122

9.  Fibroblast growth factor receptor 1 is a key regulator of early adipogenic events in human preadipocytes.

Authors:  C H Widberg; F S Newell; A W Bachmann; S N Ramnoruth; M C Spelta; J P Whitehead; L J Hutley; J B Prins
Journal:  Am J Physiol Endocrinol Metab       Date:  2008-10-21       Impact factor: 4.310

10.  Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells.

Authors:  Natsuko Kakudo; Ayuko Shimotsuma; Kenji Kusumoto
Journal:  Biochem Biophys Res Commun       Date:  2007-05-21       Impact factor: 3.575
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  3 in total

1.  Adipocytes Provide Fatty Acids to Acute Lymphoblastic Leukemia Cells.

Authors:  Jonathan Tucci; Ting Chen; Katherine Margulis; Etan Orgel; Rebecca L Paszkiewicz; Michael D Cohen; Matthew J Oberley; Rachel Wahhab; Anthony E Jones; Ajit S Divakaruni; Cheng-Chih Hsu; Sarah E Noll; Xia Sheng; Richard N Zare; Steven D Mittelman
Journal:  Front Oncol       Date:  2021-04-22       Impact factor: 6.244

2.  Advanced glycation end-products regulate extracellular matrix-adipocyte metabolic crosstalk in diabetes.

Authors:  Clarissa Strieder-Barboza; Nicki A Baker; Carmen G Flesher; Monita Karmakar; Christopher K Neeley; Dominic Polsinelli; Justin B Dimick; Jonathan F Finks; Amir A Ghaferi; Oliver A Varban; Carey N Lumeng; Robert W O'Rourke
Journal:  Sci Rep       Date:  2019-12-24       Impact factor: 4.379

3.  SARS-CoV-2 infection drives an inflammatory response in human adipose tissue through infection of adipocytes and macrophages.

Authors:  Giovanny J Martínez-Colón; Kalani Ratnasiri; Heping Chen; Sizun Jiang; Elizabeth Zanley; Arjun Rustagi; Renu Verma; Han Chen; Jason R Andrews; Kirsten D Mertz; Alexandar Tzankov; Dan Azagury; Jack Boyd; Garry P Nolan; Christian M Schürch; Matthias S Matter; Catherine A Blish; Tracey L McLaughlin
Journal:  Sci Transl Med       Date:  2022-09-22       Impact factor: 19.319
  3 in total

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