Literature DB >> 28242670

Integron-Associated DfrB4, a Previously Uncharacterized Member of the Trimethoprim-Resistant Dihydrofolate Reductase B Family, Is a Clinically Identified Emergent Source of Antibiotic Resistance.

Jacynthe L Toulouse1,2,3, Thaddeus J Edens4, Lorea Alejaldre1,2,3, Amee R Manges5, Joelle N Pelletier6,2,3,7.   

Abstract

Whole-genome sequencing of trimethoprim-resistant Escherichia coli clinical isolates identified a member of the trimethoprim-resistant type II dihydrofolate reductase gene family (dfrB). The dfrB4 gene was located within a class I integron flanked by multiple resistance genes. This arrangement was previously reported in a 130.6-kb multiresistance plasmid. The DfrB4 protein conferred a >2,000-fold increased trimethoprim resistance on overexpression in E. coli Our results are consistent with the finding that dfrB4 contributes to clinical trimethoprim resistance.
Copyright © 2017 American Society for Microbiology.

Entities:  

Keywords:  E. coli clinical isolates; antibiotic-resistant genes; class I integron; dfrB4; trimethoprim resistance; type II dihydrofolate reductase; urinary tract infection

Mesh：

Substances：

Year:  2017        PMID： 28242670      PMCID： PMC5404529          DOI： 10.1128/AAC.02665-16

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  32 in total

1.  Trimethoprim resistance in a porcine Pasteurella aerogenes isolate is based on a dfrA1 gene cassette located in a partially truncated class 2 integron.

Authors:  Corinna Kehrenberg; Stefan Schwarz
Journal:  J Antimicrob Chemother       Date:  2010-12-05       Impact factor: 5.790

2.  First detection of a carbapenem-hydrolyzing metalloenzyme in two enterobacteriaceae isolates in Spain.

Authors:  M Teresa Tórtola; Susana Lavilla; Elisenda Miró; Juan José González; Nieves Larrosa; Montserrat Sabaté; Ferran Navarro; Guillermo Prats
Journal:  Antimicrob Agents Chemother       Date:  2005-08       Impact factor: 5.191

3.  Class 1 integrons mediate antibiotic resistance in the fish pathogen Aeromonas salmonicida worldwide.

Authors:  T M L'Abée-Lund; H Sørum
Journal:  Microb Drug Resist       Date:  2001       Impact factor: 3.431

4.  New integron-associated gene cassette encoding a trimethoprim-resistant DfrB-type dihydrofolate reductase.

Authors:  Renee S Levings; Diane Lightfoot; Liam D H Elbourne; Steven P Djordjevic; Ruth M Hall
Journal:  Antimicrob Agents Chemother       Date:  2006-08       Impact factor: 5.191

5.  Protein expression in Escherichia coli minicells containing recombinant plasmids specifying trimethoprim-resistant dihydrofolate reductases.

Authors:  M E Fling; L P Elwell
Journal:  J Bacteriol       Date:  1980-02       Impact factor: 3.490

6.  Prevalence and characterization of class 1 and class 2 integrons in Escherichia coli isolated from meat and meat products of Norwegian origin.

Authors:  Marianne Sunde
Journal:  J Antimicrob Chemother       Date:  2005-10-20       Impact factor: 5.790

7.  Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides.

Authors:  Jun-ichi Wachino; Keigo Shibayama; Hiroshi Kurokawa; Kouji Kimura; Kunikazu Yamane; Satowa Suzuki; Naohiro Shibata; Yasuyoshi Ike; Yoshichika Arakawa
Journal:  Antimicrob Agents Chemother       Date:  2007-09-17       Impact factor: 5.191

8.  MOLECULAR DETECTION OF ANTIBIOTIC-RESISTANCE DETERMINANTS IN ESCHERICHIA COLI ISOLATED FROM THE ENDANGERED AUSTRALIAN SEA LION (NEOPHOCA CINEREA).

Authors:  Tiffany C Delport; Robert G Harcourt; Linda J Beaumont; Koa N Webster; Michelle L Power
Journal:  J Wildl Dis       Date:  2015-04-28       Impact factor: 1.535

9.  Occurrence of integron-associated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant.

Authors:  Thomas Tennstedt; Rafael Szczepanowski; Sebastian Braun; Alfred Pühler; Andreas Schlüter
Journal:  FEMS Microbiol Ecol       Date:  2003-08-01       Impact factor: 4.194

10.  Mechanisms of bacterial resistance to chromium compounds.

Authors:  Martha I Ramírez-Díaz; César Díaz-Pérez; Eréndira Vargas; Héctor Riveros-Rosas; Jesús Campos-García; Carlos Cervantes
Journal:  Biometals       Date:  2007-10-13       Impact factor: 2.949
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  6 in total

1.  Crowders Steal Dihydrofolate Reductase Ligands through Quinary Interactions.

Authors:  Michael R Duff; Nidhi Desai; Michael A Craig; Pratul K Agarwal; Elizabeth E Howell
Journal:  Biochemistry       Date:  2019-02-18       Impact factor: 3.162

2.  The Bacterial Genomic Context of Highly Trimethoprim-Resistant DfrB Dihydrofolate Reductases Highlights an Emerging Threat to Public Health.

Authors:  Claudèle Lemay-St-Denis; Sarah-Slim Diwan; Joelle N Pelletier
Journal:  Antibiotics (Basel)       Date:  2021-04-13

3.  Genomic analysis of trimethoprim-resistant extraintestinal pathogenic Escherichia coli and recurrent urinary tract infections.

Authors:  Dmitriy Li; Cameron J Reid; Timothy Kudinha; Veronica M Jarocki; Steven P Djordjevic
Journal:  Microb Genom       Date:  2020-11-18

4.  Small Angle Neutron Scattering Studies of R67 Dihydrofolate Reductase, a Tetrameric Protein with Intrinsically Disordered N-Termini.

Authors:  Purva P Bhojane; Michael R Duff; Khushboo Bafna; Pratul Agarwal; Christopher Stanley; Elizabeth E Howell
Journal:  Biochemistry       Date:  2017-11-07       Impact factor: 3.162

5.  Exploration into the origins and mobilization of di-hydrofolate reductase genes and the emergence of clinical resistance to trimethoprim.

Authors:  Miquel Sánchez-Osuna; Pilar Cortés; Montserrat Llagostera; Jordi Barbé; Ivan Erill
Journal:  Microb Genom       Date:  2020-11

6.  Development of antibacterial compounds that constrain evolutionary pathways to resistance.

Authors:  Yanmin Zhang; Sourav Chowdhury; João V Rodrigues; Eugene Shakhnovich
Journal:  Elife       Date:  2021-07-19       Impact factor: 8.140
  6 in total

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