J-N Liu1, Y-M Shangguan. 1. Department of Thyroid Surgery, Department of Outpatient; The Second Hospital of Shandong University, Jinan, Shandong, China. sggm19@163.com.
Abstract
OBJECTIVE: Accumulating evidence has demonstrated that microRNAs (miRNAs) play critical roles in cancer initiation and development. The present study aims to explore the clinical significance of long noncoding RNA CARLo-5 (CARLo-5) in gastric cancer. PATIENTS AND METHODS: CARLo-5 expression in normal gastric tissue, gastric cancer were quantified by Quantitative reverse transcription PCR. The relationship between CARLo-5 expression and the clinical features of gastric cancer was assessed. Kaplan-Meier method with log-rank test was applied to compare survival curves. The univariate and multivariate Cox regression models were employed to evaluate the prognostic value of the CARLo-5 expression in overall survival (OS) and relapse-free survival (RFS). RESULTS: We observed that the relative expression of CARLo-5 in gastric cancer tissues was significantly higher than that of their matched adjacent normal tissues (p < 0.001). High CARLo-5 expression was found to be closely correlated with advanced T stage (p = 0.003), positive distant metastasis (p = 0.009), lymph node involvement (p = 0.001), and poor differentiation (p = 0.001). Furthermore, Kaplan-Meier analysis demonstrated that gastric cancer patients with high CARLo-5 expression had poorer OS (p < 0.001) and RFS (p < 0.001). Finally, univariate and multivariate Cox analysis indicated that high CARLo-5 expression were independent predictors for both OS and RFS. CONCLUSIONS: Our findings implicated that CARLo-5 could be considered promising biomarkers for prognosis of gastric cancer.
OBJECTIVE: Accumulating evidence has demonstrated that microRNAs (miRNAs) play critical roles in cancer initiation and development. The present study aims to explore the clinical significance of long noncoding RNA CARLo-5 (CARLo-5) in gastric cancer. PATIENTS AND METHODS: CARLo-5 expression in normal gastric tissue, gastric cancer were quantified by Quantitative reverse transcription PCR. The relationship between CARLo-5 expression and the clinical features of gastric cancer was assessed. Kaplan-Meier method with log-rank test was applied to compare survival curves. The univariate and multivariate Cox regression models were employed to evaluate the prognostic value of the CARLo-5 expression in overall survival (OS) and relapse-free survival (RFS). RESULTS: We observed that the relative expression of CARLo-5 in gastric cancer tissues was significantly higher than that of their matched adjacent normal tissues (p < 0.001). High CARLo-5 expression was found to be closely correlated with advanced T stage (p = 0.003), positive distant metastasis (p = 0.009), lymph node involvement (p = 0.001), and poor differentiation (p = 0.001). Furthermore, Kaplan-Meier analysis demonstrated that gastric cancerpatients with high CARLo-5 expression had poorer OS (p < 0.001) and RFS (p < 0.001). Finally, univariate and multivariate Cox analysis indicated that high CARLo-5 expression were independent predictors for both OS and RFS. CONCLUSIONS: Our findings implicated that CARLo-5 could be considered promising biomarkers for prognosis of gastric cancer.