Literature DB >> 2823639

An assay of collagenase activity using enzyme-linked immunosorbent assay for mammalian collagenase.

H Yoshioka1, I Oyamada, G Usuku.   

Abstract

A new method was developed for the measurement of collagenase activity using the enzyme-linked immunosorbent assay (ELISA). Rabbit colon wall collagenase, pepsin-soluble rat skin type I collagen, and its antisera were used in the present experiment. After the collagenase-degraded portion of the collagen coated on the microwell was released, the immunoreaction of the residual collagen on the microwell to anticollagen sera was determined by ELISA. This method was approximately 10 times more sensitive than the conventional assay procedure using [14C]-glycine-labeled reconstituted collagen fibrils as substrate. It was suitable for screening a large number of samples without radioisotopes.

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Year:  1987        PMID： 2823639     DOI： 10.1016/0003-2697(87)90559-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Cytokines in chronic inflammatory arthritis. V. Mutual antagonism between interferon-gamma and tumor necrosis factor-alpha on HLA-DR expression, proliferation, collagenase production, and granulocyte macrophage colony-stimulating factor production by rheumatoid arthritis synoviocytes.

Authors:  J M Alvaro-Gracia; N J Zvaifler; G S Firestein
Journal:  J Clin Invest       Date:  1990-12       Impact factor: 14.808

2.  Comparison of techniques for measurement of gelatinases/type IV collagenases: enzyme-linked immunoassays versus substrate degradation assays.

Authors:  S Zucker; P Mancuso; B DiMassimo; R M Lysik; C Conner; C L Wu
Journal:  Clin Exp Metastasis       Date:  1994-01       Impact factor: 5.150

3.  Safety Evaluation and Anti-wrinkle Effects of Retinoids on Skin.

Authors:  Bae-Hwan Kim
Journal:  Toxicol Res       Date:  2010-03
  3 in total

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