| Literature DB >> 2823634 |
J J Kupiec1, M L Giron, D Vilette, J M Jeltsch, R Emanoil-Ravier.
Abstract
A new method for the isolation of high-molecular-weight DNA from eukaryotic cells is presented. It is based on formamide treatment and extensive dialysis of cellular proteinase K digests. This procedure consists mainly of a series of incubations allowing simultaneous preparation of several DNAs which can then be restricted, ligated, hybridized, and cloned.Entities:
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Year: 1987 PMID： 2823634 DOI： 10.1016/0003-2697(87)90366-6
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365