Literature DB >> 2823134

Trypanosoma cruzi isolates from Argentina and Chile grouped with the aid of DNA probes.

R A Macina1, S Arauzo, M B Reyes, D O Sanchez, M A Basombrio, E E Montamat, A Solari, A C Frasch.   

Abstract

Fifty-two isolates and several clones from Trypanosoma cruzi, the agent of Chagas' disease, were analyzed using cloned minicircles or total kinetoplast DNA as probes. Isolates were obtained from triatomines, guinea pigs and infected humans in the Central and Northern regions of Argentina and the North of Chile. 35% of all the randomly selected isolates could be identified with one cloned minicircle probe. This widely distributed T. cruzi group was detected on both sides of the Andes mountain range (Argentina and Chile) in Triatoma infestans as well as in human infections. Most of the other isolates could be grouped with four kinetoplast DNAs as probes, but their geographical distribution seems to be restricted as compared with the one mentioned above. These results confirm the heterogeneity of T. cruzi subspecies in nature and the usefulness of DNA probes to group them.

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Year:  1987        PMID： 2823134     DOI： 10.1016/0166-6851(87)90017-x

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN： 0166-6851            Impact factor:   1.759


  7 in total

Review 1.  [Molecular biological techniques in the diagnosis of tropical parasitic diseases].

Authors:  R Felleisen; M Q Klinkert
Journal:  Naturwissenschaften       Date:  1992-11

2.  Trypanosoma cruzi I genotypes in different geographical regions and transmission cycles based on a microsatellite motif of the intergenic spacer of spliced-leader genes.

Authors:  Carolina I Cura; Ana M Mejía-Jaramillo; Tomás Duffy; Juan M Burgos; Marcela Rodriguero; Marta V Cardinal; Sonia Kjos; Rodrigo Gurgel-Gonçalves; Denis Blanchet; Luis M De Pablos; Nicolás Tomasini; Alexandre da Silva; Graciela Russomando; Cesar A Cuba Cuba; Christine Aznar; Teresa Abate; Mariano J Levin; Antonio Osuna; Ricardo E Gürtler; Patricio Diosque; Aldo Solari; Omar Triana-Chávez; Alejandro G Schijman
Journal:  Int J Parasitol       Date:  2010-07-27       Impact factor: 3.981

3.  DNA probes specific for Entamoeba histolytica possessing pathogenic and nonpathogenic zymodemes.

Authors:  L I Garfinkel; M Giladi; M Huber; C Gitler; D Mirelman; M Revel; S Rozenblatt
Journal:  Infect Immun       Date:  1989-03       Impact factor: 3.441

4.  Characterization and grouping of Trypanosoma cruzi stocks by DNA base-specific fluorochromes and discriminant analysis.

Authors:  H Mühlpfordt; J Berger
Journal:  Parasitol Res       Date:  1990       Impact factor: 2.289

5.  Trypanosoma cruzi genotypes of insect vectors and patients with Chagas of Chile studied by means of cytochrome b gene sequencing, minicircle hybridization, and nuclear gene polymorphisms.

Authors:  Marco Arenas; Ricardo Campos; Ximena Coronado; Sylvia Ortiz; Aldo Solari
Journal:  Vector Borne Zoonotic Dis       Date:  2011-10-24       Impact factor: 2.133

6.  Genomic DNA differences between pathogenic and nonpathogenic Entamoeba histolytica.

Authors:  E Tannich; R D Horstmann; J Knobloch; H H Arnold
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

7.  Multiplex Real-Time PCR Assay Using TaqMan Probes for the Identification of Trypanosoma cruzi DTUs in Biological and Clinical Samples.

Authors:  Carolina I Cura; Tomas Duffy; Raúl H Lucero; Margarita Bisio; Julie Péneau; Matilde Jimenez-Coello; Eva Calabuig; María J Gimenez; Edward Valencia Ayala; Sonia A Kjos; José Santalla; Susan M Mahaney; Nelly M Cayo; Claudia Nagel; Laura Barcán; Edith S Málaga Machaca; Karla Y Acosta Viana; Laurent Brutus; Susana B Ocampo; Christine Aznar; Cesar A Cuba Cuba; Ricardo E Gürtler; Janine M Ramsey; Isabela Ribeiro; John L VandeBerg; Zaida E Yadon; Antonio Osuna; Alejandro G Schijman
Journal:  PLoS Negl Trop Dis       Date:  2015-05-19
  7 in total

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