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Literature DB >> 2823127

Developmental regulation and identification of an isotype encoded by altB, an alpha-tubulin locus in Physarum polycephalum.

L L Green¹, M M Schroeder, M A Diggins, W F Dove.

Abstract

A subcloned portion of the 5' nontranslated sequence from a Physarum alpha-tubulin cDNA is specific for a single alpha-tubulin locus, altB, of Physarum polycephalum. We find that this locus is expressed only in the plasmodium and encodes at least an alpha 1-tubulin isotype, which we have designated alpha 1B. Hybridization patterns of other subclones of this cDNA reveal two sequences for alpha-tubulin at the altB locus.

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Year: 1987 PMID： 2823127 PMCID： PMC367974 DOI： 10.1128/mcb.7.9.3337-3340.1987

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

22 in total

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

2. Demonstration of different patterns of microtubule organization in Physarum polycephalum myxamoebae and plasmodia using immunofluorescence microscopy.

Authors: J C Havercroft; K Gull
Journal: Eur J Cell Biol Date: 1983-11 Impact factor: 4.492

2. Two alleles of a developmentally regulated alpha-tubulin locus in Physarum polycephalum replicate on different schedules.

Authors: D B Cunningham; W F Dove
Journal: Mol Cell Biol Date: 1993-01 Impact factor: 4.272

2 in total

Developmental regulation and identification of an isotype encoded by altB, an alpha-tubulin locus in Physarum polycephalum.

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

2. Demonstration of different patterns of microtubule organization in Physarum polycephalum myxamoebae and plasmodia using immunofluorescence microscopy.

3. Quantitative isolation of DNA restriction fragments from low-melting agarose by Elutip-d affinity chromatography.

4. Genetic analysis of resistance to benzimidazoles in Physarum: differential expression of beta-tubulin genes.

5. Patterns of tubulin isotype synthesis and usage during mitotic spindle morphogenesis in Physarum.

6. Identification of tubulin isoforms in the plasmodium of Physarum polycephalum by in vitro microtubule assembly.

7. A plasmodial alpha-tubulin cDNA from Physarum polycephalum. Nucleotide sequence and comparative analysis.

8. Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum.

9. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.

10. Cell type-dependent expression of tubulins in Physarum.

Review 1. Microtubules in Microorganisms: How Tubulin Isotypes Contribute to Diverse Cytoskeletal Functions.

2. Two alleles of a developmentally regulated alpha-tubulin locus in Physarum polycephalum replicate on different schedules.