Literature DB >> 28229403

A Simple Protocol to Isolate, Characterize, and Expand Dental Pulp Stem Cells.

Federica Di Scipio1, Andrea Elio Sprio1, Maria Elisabetta Carere1, Zhiqian Yang1, Giovanni Nicolao Berta2.   

Abstract

Adult stem cells reside in body tissues to preserve organs and whole organism homeostasis. They are acquiring a prominent role in the contemporary medicine. Many protocols to isolate and cultivate adult stem cells have been so far described, though they are often lengthy, laborious, and require very expansive instruments, materials, and reagents. On this basis, we describe a simple, cheap but at the same time functional method to: (1) isolate dental pulp stem cells (DPSC), (2) expand and cultivate DPSC, (3) cryopreserve DPSC, (4) characterize DPSC, and (5) differentiate DPSC into both mesenchymal and non-mesenchymal lineages.

Entities:  

Keywords:  Cell extraction; Characterization; Dental pulp stem cells; Differentiation; Expansion; Neural crest

Mesh：

Substances：

Year:  2017        PMID： 28229403     DOI： 10.1007/978-1-4939-6756-8_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

Review 1.  Stem Cell-Based Therapies: What Interventional Radiologists Need to Know.

Authors:  Hyeon Yu; Clayton W Commander; Joseph M Stavas
Journal:  Semin Intervent Radiol       Date:  2021-11-24       Impact factor: 1.780

Review 2.  Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration.

Authors:  Sarah Hani Shoushrah; Janis Lisa Transfeld; Christian Horst Tonk; Dominik Büchner; Steffen Witzleben; Martin A Sieber; Margit Schulze; Edda Tobiasch
Journal:  Int J Mol Sci       Date:  2021-06-15       Impact factor: 5.923

3.  Therapeutic effect of dental pulp stem cell transplantation on a rat model of radioactivity-induced esophageal injury.

Authors:  Chunwei Zhang; Yichi Zhang; Zhenning Feng; Feifei Zhang; Zishuai Liu; Xiaoli Sun; Mengting Ruan; Mingna Liu; Shizhu Jin
Journal:  Cell Death Dis       Date:  2018-07-03       Impact factor: 8.469

4.  Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis.

Authors:  Ning Li; Yichi Zhang; Narayan Nepal; Guoqing Li; Ningning Yang; Haoyuan Chen; Qiuchi Lin; Xuechun Ji; Sijia Zhang; Shizhu Jin
Journal:  Stem Cell Res Ther       Date:  2021-01-07       Impact factor: 6.832

5.  The effect of implants loaded with stem cells from human exfoliated deciduous teeth on early osseointegration in a canine model.

Authors:  Xu Cao; Caiyun Wang; Dingxiang Yuan; Su Chen; Xin Wang
Journal:  BMC Oral Health       Date:  2022-06-17       Impact factor: 3.747

6.  Effect of Ascorbic Acid on Differentiation, Secretome and Stemness of Stem Cells from Human Exfoliated Deciduous Tooth (SHEDs).

Authors:  Shilpa Bhandi; Ahmed Alkahtani; Mohammed Mashyakhy; Abdulaziz S Abumelha; Nassreen Hassan Mohammad Albar; Apathsakayan Renugalakshmi; Mazen F Alkahtany; Ali Robaian; Asma Saleh Almeslet; Vikrant R Patil; Saranya Varadarajan; Thodur Madapusi Balaji; Rodolfo Reda; Luca Testarelli; Shankargouda Patil
Journal:  J Pers Med       Date:  2021-06-22
  6 in total

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