| Literature DB >> 2822653 |
T Fujii1, D Naka, N Toyoda, H Seto.
Abstract
When cells competent for genetic transformation of Streptococcus pneumoniae which could bind and enable entry of extracellular DNA molecules were treated with LiCl, they released a nickase that introduced nicks into a double-stranded DNA in the presence of EDTA. The nickase was specific for competent cells and coupled with DNA-binding activity. Furthermore, when noncompetent cells were treated with LiCl, they released the putative receptors for the competence activator.Entities:
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Year: 1987 PMID： 2822653 PMCID： PMC213883 DOI： 10.1128/jb.169.11.4901-4906.1987
Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490