Literature DB >> 28224224

Aminopeptidase N5 (APN5) as a Putative Functional Receptor of Cry1Ac Toxin in the Larvae of Athetis lepigone.

Li-Yu Wang1, Shao-Hua Gu2, Zi-Yan Nangong1, Ping Song3, Qin-Ying Wang1.   

Abstract

Athetis lepigone was a new lepidopteran pest and caused severe damage to maize crops in China. We have detected that Cry1Ac protoxin and toxin were highly active against the larvae of A. lepigone. However, there is no report about the mode of action of Bt Cry1Ac toxin against this pest until now. A 110 kDa APN5 protein from BBMV of A. lepigone was identified as the binding receptor of Cry1Ac toxin using Ligand blotting. The Cry1Ac receptor APN5 was cloned from A. lepigone larval midgut mRNA and named as AlAPN5 (GenBank accession no.: KU950745). AlAPN5 had a GATEN motif and been classified to Class 5 APNs. 79.2% reduction in mortality was observed when A. lepigone larvae were injected with siRNA of the AlAPN5 gene and treated with Cry1Ac toxin. These data demonstrate that AlAPN5 is a putative functional receptor and maybe the only receptor of Cry1Ac in A. lepigone.

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Year:  2017        PMID： 28224224     DOI： 10.1007/s00284-017-1215-0

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  21 in total

1.  Bacillus thuringiensis Cry1Ac and Cry1Fa delta-endotoxin binding to a novel 110 kDa aminopeptidase in Heliothis virescens is not N-acetylgalactosamine mediated.

Authors:  D J Banks; J L Jurat-Fuentes; D H Dean; M J Adang
Journal:  Insect Biochem Mol Biol       Date:  2001-07-26       Impact factor: 4.714

2.  Identification of Henosepilachna vigintioctomaculata (Coleoptera: Coccinellidae) midgut putative receptor for Bacillus thuringiensis insecticidal Cry7Ab3 toxin.

Authors:  Ping Song; QinYing Wang; ZiYan Nangong; JunPing Su; DongHua Ge
Journal:  J Invertebr Pathol       Date:  2012-01-27       Impact factor: 2.841

Review 3.  Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control.

Authors:  Alejandra Bravo; Sarjeet S Gill; Mario Soberón
Journal:  Toxicon       Date:  2006-11-30       Impact factor: 3.033

Review 4.  Role of receptors in Bacillus thuringiensis crystal toxin activity.

Authors:  Craig R Pigott; David J Ellar
Journal:  Microbiol Mol Biol Rev       Date:  2007-06       Impact factor: 11.056

Review 5.  Why Bacillus thuringiensis insecticidal toxins are so effective: unique features of their mode of action.

Authors:  A I Aronson; Y Shai
Journal:  FEMS Microbiol Lett       Date:  2001-02-05       Impact factor: 2.742

6.  Silencing of midgut aminopeptidase N of Spodoptera litura by double-stranded RNA establishes its role as Bacillus thuringiensis toxin receptor.

Authors:  R Rajagopal; S Sivakumar; Neema Agrawal; Pawan Malhotra; Raj K Bhatnagar
Journal:  J Biol Chem       Date:  2002-10-10       Impact factor: 5.157

7.  Aminopeptidase N isoforms from the midgut of Bombyx mori and Plutella xylostella -- their classification and the factors that determine their binding specificity to Bacillus thuringiensis Cry1A toxin.

Authors:  Kazuko Nakanishi; Katsuro Yaoi; Yasushi Nagino; Hirotaka Hara; Madoka Kitami; Shogo Atsumi; Nami Miura; Ryoichi Sato
Journal:  FEBS Lett       Date:  2002-05-22       Impact factor: 4.124

8.  Identification and characterization of Aedes aegypti aminopeptidase N as a putative receptor of Bacillus thuringiensis Cry11A toxin.

Authors:  Jianwu Chen; Karlygash G Aimanova; Songqin Pan; Sarjeet S Gill
Journal:  Insect Biochem Mol Biol       Date:  2009-08-19       Impact factor: 4.714

9.  Purification and partial amino acid sequences of the binding protein from Bombyx mori for CryIAa delta-endotoxin of Bacillus thuringiensis.

Authors:  H Ihara; T Uemura; M Masuhara; S Ikawa; K Sugimoto; A Wadano; M Himeno
Journal:  Comp Biochem Physiol B Biochem Mol Biol       Date:  1998-05       Impact factor: 2.231

10.  Identification of ABCC2 as a binding protein of Cry1Ac on brush border membrane vesicles from Helicoverpa armigera by an improved pull-down assay.

Authors:  Zishan Zhou; Zeyu Wang; Yuxiao Liu; Gemei Liang; Changlong Shu; Fuping Song; Xueping Zhou; Alejandra Bravo; Mario Soberón; Jie Zhang
Journal:  Microbiologyopen       Date:  2016-04-01       Impact factor: 3.139
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