BACKGROUND/AIMS: This study investigated the effect of mechanical stress on tendon-bone healing in a rabbit anterior cruciate ligament (ACL) reconstruction model as well as cell proliferation and matrix formation in co-culture of bone-marrow mesenchymal stem cells (BMSCs) and tendon cells (TCs). METHODS: The effect of continuous passive motion (CPM) therapy on tendon-bone healing in a rabbit ACL reconstruction model was evaluated by histological analysis, biomechanical testing and gene expressions at the tendon-bone interface. Furthermore, the effect of mechanical stretch on cell proliferation and matrix synthesis in BMSC/TC co-culture was also examined. RESULTS: Postoperative CPM therapy significantly enhanced tendon-bone healing, as evidenced by increased amount of fibrocartilage, elevated ultimate load to failure levels, and up-regulated gene expressions of Collagen I, alkaline phosphatase, osteopontin, Tenascin C and tenomodulin at the tendon-bone junction. In addition, BMSC/TC co-culture treated with mechanical stretch showed a higher rate of cell proliferation and enhanced expressions of Collagen I, Collagen III, alkaline phosphatase, osteopontin, Tenascin C and tenomodulin than that of controls. CONCLUSION: These results demonstrated that proliferation and differentiation of local precursor cells could be enhanced by mechanical stimulation, which results in enhanced regenerative potential of BMSCs and TCs in tendon-bone healing.
BACKGROUND/AIMS: This study investigated the effect of mechanical stress on tendon-bone healing in a rabbit anterior cruciate ligament (ACL) reconstruction model as well as cell proliferation and matrix formation in co-culture of bone-marrow mesenchymal stem cells (BMSCs) and tendon cells (TCs). METHODS: The effect of continuous passive motion (CPM) therapy on tendon-bone healing in a rabbit ACL reconstruction model was evaluated by histological analysis, biomechanical testing and gene expressions at the tendon-bone interface. Furthermore, the effect of mechanical stretch on cell proliferation and matrix synthesis in BMSC/TC co-culture was also examined. RESULTS: Postoperative CPM therapy significantly enhanced tendon-bone healing, as evidenced by increased amount of fibrocartilage, elevated ultimate load to failure levels, and up-regulated gene expressions of Collagen I, alkaline phosphatase, osteopontin, Tenascin C and tenomodulin at the tendon-bone junction. In addition, BMSC/TC co-culture treated with mechanical stretch showed a higher rate of cell proliferation and enhanced expressions of Collagen I, Collagen III, alkaline phosphatase, osteopontin, Tenascin C and tenomodulin than that of controls. CONCLUSION: These results demonstrated that proliferation and differentiation of local precursor cells could be enhanced by mechanical stimulation, which results in enhanced regenerative potential of BMSCs and TCs in tendon-bone healing.
Authors: Xi Chen; Jinku Yan; Fan He; Dongyan Zhong; Huilin Yang; Ming Pei; Zong-Ping Luo Journal: Free Radic Biol Med Date: 2018-08-07 Impact factor: 7.376
Authors: Angelica Marie van Gogh; Xingguang Li; Gun Min Youn; Alyssa Alvarez; Sophia Yin; Moyukh O Chakrabarti; Patrick J McGahan; James L Chen Journal: Arthrosc Tech Date: 2019-12-18
Authors: Xaver Feichtinger; Patrick Heimel; Stefan Tangl; Claudia Keibl; Sylvia Nürnberger; Jakob Emanuel Schanda; David Hercher; Roland Kocijan; Heinz Redl; Johannes Grillari; Christian Fialka; Rainer Mittermayr Journal: PLoS One Date: 2022-01-05 Impact factor: 3.240
Authors: Adam T Hexter; Aikaterina Karali; Alex Kao; Gianluca Tozzi; Nima Heidari; Aviva Petrie; Ashleigh Boyd; Deepak M Kalaskar; Catherine Pendegrass; Scott Rodeo; Fares Haddad; Gordon Blunn Journal: Orthop J Sports Med Date: 2021-09-21