Literature DB >> 2821185

Quantitative immunocytochemical assay for infectious avian retroviruses.

A W Stoker1, M J Bissell.   

Abstract

A simple and accurate immunocytochemical focus assay is described, whereby both transforming and non-transforming avian retroviruses can be enumerated. After virus infection of chick embryo fibroblasts, an agar overlay is applied; foci of infected cells (expression foci) are detected immunocytochemically after 5 to 7 days. The primary antibodies are monoclonal sera directed against either viral p19gag or pp60v-src. Detection of expression foci after transfection of cells with cloned viral DNA is also demonstrated.

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Year:  1987        PMID： 2821185     DOI： 10.1099/0022-1317-68-9-2481

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  22 in total

1.  A novel interferon regulatory factor (IRF), IRF-10, has a unique role in immune defense and is induced by the v-Rel oncoprotein.

Authors:  Jirí Nehyba; Radmila Hrdlicková; Joan Burnside; Henry R Bose
Journal:  Mol Cell Biol       Date:  2002-06       Impact factor: 4.272

2.  Development of avian sarcoma and leukosis virus-based vector-packaging cell lines.

Authors:  A W Stoker; M J Bissell
Journal:  J Virol       Date:  1988-03       Impact factor: 5.103

3.  A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina.

Authors:  S C Fields-Berry; A L Halliday; C L Cepko
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205

4.  Transduction of proto-src sequences in tissue culture by a molecular clone of transformation-defective Rous sarcoma virus with an internal src deletion.

Authors:  W Phares
Journal:  J Virol       Date:  1988-12       Impact factor: 5.103

5.  MRI-based detection of alkaline phosphatase gene reporter activity using a porphyrin solubility switch.

Authors:  Gil G Westmeyer; Yelena Emer; Jutta Lintelmann; Alan Jasanoff
Journal:  Chem Biol       Date:  2014-03-06

Review 6.  Vectors derived from avian leukosis and sarcoma viruses.

Authors:  F Flamant; F L Cosset; J Samarut
Journal:  J Mol Med (Berl)       Date:  1995-04       Impact factor: 4.599

7.  Construction and characterization of a highly complex retroviral library for lineage analysis.

Authors:  J A Golden; S C Fields-Berry; C L Cepko
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-06       Impact factor: 11.205

8.  Evolution of the oncogenic potential of v-rel: rel-induced expression of immunoregulatory receptors correlates with tumor development and in vitro transformation.

Authors:  J Nehyba; R Hrdlicková; E H Humphries
Journal:  J Virol       Date:  1994-04       Impact factor: 5.103

9.  The relocalization of v-Rel from the nucleus to the cytoplasm coincides with induction of expression of Ikba and nfkb1 and stabilization of I kappa B-alpha.

Authors:  R Hrdlicková; J Nehyba; A Roy; E H Humphries; H R Bose
Journal:  J Virol       Date:  1995-01       Impact factor: 5.103

10.  v-src induces clonal sarcomas and rapid metastasis following transduction with a replication-defective retrovirus.

Authors:  A W Stoker; M H Sieweke
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205
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