Literature DB >> 2821044

Single-step procedure for the extraction and purification of leukotrienes B4, C4 and D4.

R Richmond1, N C Turner, N Maltby, D Heavey, J Vial, C T Dollery, G W Taylor.   

Abstract

A rapid and simple method for the on-line concentration and high-performance liquid chromatographic (HPLC) purification of the leukotrienes in good yield from biological fluids is described. Readily available antisera are used in conjunction with this system to give a specific and sensitive assay for leukotrienes B4, C4 and D4 with sub-nanogram limits of detection. Tritium-labelled leukotrienes are used as internal standards, both to locate the leukotrienes post-HPLC and to accurately determine recoveries.

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Year:  1987        PMID： 2821044     DOI： 10.1016/0378-4347(87)80117-2

Source DB:  PubMed          Journal:  J Chromatogr


  2 in total

1.  Effect of a 5-lipoxygenase inhibitor on leukotriene generation and airway responses after allergen challenge in asthmatic patients.

Authors:  K P Hui; I K Taylor; G W Taylor; P Rubin; J Kesterson; N C Barnes; P J Barnes
Journal:  Thorax       Date:  1991-03       Impact factor: 9.139

2.  Release of arachidonic acid metabolites and histamine from sensitized guinea-pig lung following antigen challenge.

Authors:  N C Turner; C T Dollery
Journal:  Br J Pharmacol       Date:  1988-04       Impact factor: 8.739
  2 in total

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